deoD

168

purine nucleoside phosphorylase

Locus: BSU_19630

Molecular weight: 25.23 kDa

Isoelectric point: 4.98

Protein length: 233 aa

Gene length: 702 bp

Function: purine salvage and interconversion

Product: purine nucleoside phosphorylase

Essential: no

E.C.: 2.4.2.1

Synonyms: deoD

Genomic Context

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List of homologs in different organisms, belongs to COG0813 (Galperin et al., 2021)

Gene

Coordinates: 2,135,470  2,136,171

The protein

Catalyzed reaction/ biological activity

• purine D-ribonucleoside + phosphate --> purine nucleobase + α-D-ribose 1-phosphate (according to UniProt)
• purine 2'-deoxy-D-ribonucleoside + phosphate --> 2-deoxy-α-D-ribose 1-phosphate + purine nucleobase (according to UniProt)

Protein family

• PNP/UDP phosphorylase family (with MtnN, according to UniProt)

Structure

• 4D8V (PDB) PubMed
• O34925 (AlphaFold)

Expression and Regulation

Operons

• Genes: deoD-ldcB

  Description: PubMed

Biological materials

Mutant

• BKE19630 (deoD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCATATCCTCCTGTAA,  downstream forward: _UP4_TAAAATATATCAAGAGGCGT
• BKK19630 (deoD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCATATCCTCCTGTAA,  downstream forward: _UP4_TAAAATATATCAAGAGGCGT

References

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