sacC

168

levanase

Locus: BSU_27030

Molecular weight: 75.77 kDa

Isoelectric point: 6.79

Protein length: 677 aa

Gene length: 2034 bp

Function: degradation of levan to fructose

Product: levanase

Essential: no

E.C.: 3.2.1.80

Synonyms: sacC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1621 (Galperin et al., 2021)

Gene

Coordinates: 2,758,043  2,760,076

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of terminal, non-reducing (2->1)- and (2->6)-linked beta-D-fructofuranose residues in fructans (according to UniProt)

Protein family

• glycosyl hydrolase 32 family (with LevB and SacA, according to UniProt)

Domains

• glycosyl hydrolase 32 family domain, aa 30 - 513 PubMed
• C-terminal carbohydrate-binding module, aa 515-677 PubMed

Structure

• 1Y9M (PDB) (inulinase of Aspergillus awamori, glycosyl hydrolase 32 family domain, aa 30 - 513, 42% identity) PubMed
• 4B1M (PDB) (the C-terminal carbohydrate-binding module, aa 515-677) PubMed
• P05656 (AlphaFold)

Expression and Regulation

Operons

• Genes: levD-levE-levF-levG-sacC

  Description: PubMed

  Regulation

  • induced in the presence of fructose (LevR) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon
  • LevR: activation, PubMed, in levR regulon

  Sigma factors

  • SigL: sigma factor, PubMed, in sigL regulon

Biological materials

Mutant

• BKE27030 (sacC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGTTCCTTCGCTCCTC,  downstream forward: _UP4_TAATTTTAAAAAAGGCACAG
• BKK27030 (sacC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGTTCCTTCGCTCCTC,  downstream forward: _UP4_TAATTTTAAAAAAGGCACAG

References

Reviews

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Research papers

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