levD

168

fructose-specific permease of the phosphotransferase system, EIIA of the PTS

Locus: BSU_27070

Molecular weight: 16.11 kDa

Isoelectric point: 4.48

Protein length: 146 aa

Gene length: 441 bp

Function: fructose uptake and phosphorylation

Product: fructose-specific PTS, EIIA component

Essential: no

E.C.: 2.7.1.69

Synonyms: levD

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2893 (Galperin et al., 2021)

Gene

Coordinates: 2,762,395  2,762,835

The protein

Protein family

• PTS permease, mannose family PubMed

Domains

• PTS EIIA domain type-4 (aa 1-124) (according to UniProt)

Structure

• 1PDO (PDB) (E. coli ManX, 33% identity) PubMed
• P26379 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: levD-levE-levF-levG-sacC

  Description: PubMed

  Regulation

  • induced in the presence of fructose (LevR) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon
  • LevR: activation, PubMed, in levR regulon

  Sigma factors

  • SigL: sigma factor, PubMed, in sigL regulon

Biological materials

Mutant

• BKE27070 (levD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCTATTGCTCCTTTCC,  downstream forward: _UP4_GATACAAGAGAGGATGAATT
• BKK27070 (levD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCTATTGCTCCTTTCC,  downstream forward: _UP4_GATACAAGAGAGGATGAATT

References

Loading