spoVAC

168

required for the uptake of the 1:1 chelate of pyridine-2,6-dicarboxylic acid (DPA(2,6)) and Ca(2 ) into developing spores and release during Germination, required for spore maturation

Locus: BSU_23420

Molecular weight: 15.97 kDa

Isoelectric point: 9.46

Protein length: 150 aa

Gene length: 453 bp

Function: spore maturation

Product: subunit of dipicolinate uptake complex

Essential: no

Synonyms: spoVAC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG5836 (Galperin et al., 2021)

Gene

Coordinates: 2,441,804 → 2,442,256

The protein

Catalyzed reaction/ biological activity

• forms a membrane channel with SpoVAEB PubMed
• forms a membrane complex with SpoVAEB and SpoVAD in vitro PubMed

Structure

• P40868 (AlphaFold)

Localization

• inner spore membrane PubMed

Additional information

• stability in spores depends on SpoVAEB PubMed

Expression and Regulation

Operons

• Genes: spoVAA-spoVAB-spoVAC-spoVAD-spoVAEB-spoVAEA-spoVAF-lysA

  Description: PubMed

  Regulation

  • expressed late during sporulation in the forespore (SigG, SpoVT) PubMed

  Regulatory mechanism

  • SpoVT: activation, PubMed, in spoVT regulon

  Sigma factors

  • SigG: sigma factor, PubMed, in sigG regulon

Biological materials

Mutant

• BKE23420 (ΔspoVAC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTGAAACCTCCTTATG,  downstream forward: _UP4_CTGATGTCATAGGAGGAGAA
• BKK23420 (ΔspoVAC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTGAAACCTCCTTATG,  downstream forward: _UP4_CTGATGTCATAGGAGGAGAA

Labs working on this gene/protein

• Peter Setlow, University of Connecticut Health Center, USA

References

Reviews

Loading

Original Publications

Loading