Difference between revisions of "TagT"
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− | * '''Description:''' | + | * '''Description:''' phosphotransferase, attaches teichoic acid, teichuronic acid, or acidic capsular polysaccharide to cell wall peptidoglycan via phosphodiester linkage to N-acteylmuramic acid <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || phosphotransferase, responsible for attachment of anionic polymers to peptidoglycan |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan |
+ | |- | ||
+ | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35840 tagT] | ||
+ | |- | ||
+ | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=TagT TagT] | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 9.554 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 9.554 | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywtG]]'', ''[[ywtE]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywtG]]'', ''[[ywtE]]'' | ||
|- | |- | ||
− | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU35840 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU35840 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU35840 DNA_with_flanks] |
|- | |- | ||
|- | |- | ||
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|colspan="2" | '''Genetic context''' <br/> [[Image:ywtF_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:ywtF_context.gif]] | ||
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | ||
+ | |- | ||
+ | |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywtF_3694239_3695207_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:tagT_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35840]] | ||
|- | |- | ||
|} | |} | ||
__TOC__ | __TOC__ | ||
+ | <br/><br/><br/><br/> | ||
+ | <br/><br/><br/><br/> | ||
+ | <br/><br/><br/><br/> | ||
+ | <br/><br/> | ||
+ | |||
+ | = [[Categories]] containing this gene/protein = | ||
+ | {{SubtiWiki category|[[cell wall synthesis]]}}, | ||
+ | {{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}}, | ||
+ | {{SubtiWiki category|[[membrane proteins]]}} | ||
− | + | = This gene is a member of the following [[regulons]] = | |
+ | {{SubtiWiki regulon|[[SigM regulon]]}} | ||
=The gene= | =The gene= | ||
Line 40: | Line 56: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
+ | * a ''[[tagT]]'' mutant has no phenoype, the triple ''[[tagT]] [[tagU]] [[tagV]]'' mutant is unable to grow under normal conditions {{PubMed|21964069}} | ||
=== Database entries === | === Database entries === | ||
+ | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35840&redirect=T BSU35840] | ||
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywtF.html] | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywtF.html] | ||
Line 56: | Line 74: | ||
* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
− | * '''Protein family:''' | + | * '''Protein family:''' LytR-Cps2a-Psr family {{PubMed|19099556}} |
− | * '''Paralogous protein(s):''' | + | * '''Paralogous protein(s):''' [[TagU]], [[TagV]] |
=== Extended information on the protein === | === Extended information on the protein === | ||
Line 68: | Line 86: | ||
* '''Modification:''' | * '''Modification:''' | ||
− | * '''Cofactor(s):''' | + | * '''Cofactor(s):''' Mg(2+) {{PubMed|21964069}} |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
− | * '''Interactions:''' | + | * '''[[SubtInteract|Interactions]]:''' |
+ | ** [[MreB]]-[[TagT]] {{PubMed|21964069}} | ||
− | * '''Localization:''' | + | * '''[[Localization]]:''' |
+ | ** cytoplasmic membrane (with extracytoplasmic catalaytic domain) {{PubMed|21964069}} | ||
+ | ** extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed] | ||
=== Database entries === | === Database entries === | ||
+ | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35840&redirect=T BSU35840] | ||
− | * '''Structure:''' | + | * '''Structure:''' |
+ | ** [http://www.pdb.org/pdb/explore/explore.do?structureId=3MEJ 3MEJ] | ||
+ | ** [http://www.rcsb.org/pdb/explore/explore.do?structureId=4DE9 4DE9] {{PubMed|22432711}} | ||
− | * ''' | + | * '''UniProt:''' [http://www.uniprot.org/uniprot/Q7WY78 Q7WY78] |
− | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu | + | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU35840] |
* '''E.C. number:''' | * '''E.C. number:''' | ||
Line 90: | Line 114: | ||
=Expression and regulation= | =Expression and regulation= | ||
− | * '''Operon:''' | + | * '''Operon:''' ''ywtF'' {{PubMed|9353933}} |
+ | |||
+ | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywtF_3694239_3695207_1 tagT] {{PubMed|22383849}} | ||
* '''[[Sigma factor]]:''' [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 Pubmed] | * '''[[Sigma factor]]:''' [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 Pubmed] | ||
* '''Regulation:''' | * '''Regulation:''' | ||
+ | ** expressed at high salt concentrations ([[SigM]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 Pubmed] | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
* '''Additional information:''' | * '''Additional information:''' | ||
+ | ** number of protein molecules per cell (minimal medium with glucose and ammonium): 105 {{PubMed|24696501}} | ||
+ | ** number of protein molecules per cell (complex medium with amino acids, without glucose): 258 {{PubMed|24696501}} | ||
=Biological materials = | =Biological materials = | ||
Line 119: | Line 148: | ||
=References= | =References= | ||
+ | ==Reviews== | ||
+ | <pubmed> 24024634 </pubmed> | ||
+ | ==Original publications== | ||
+ | <pubmed>18957862, 9353933 18179421 19099556 21964069 22432711</pubmed> | ||
− | + | [[Category:Protein-coding genes]] |
Latest revision as of 11:45, 19 June 2014
- Description: phosphotransferase, attaches teichoic acid, teichuronic acid, or acidic capsular polysaccharide to cell wall peptidoglycan via phosphodiester linkage to N-acteylmuramic acid
Gene name | ywtF |
Synonyms | |
Essential | no |
Product | phosphotransferase, responsible for attachment of anionic polymers to peptidoglycan |
Function | transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan |
Gene expression levels in SubtiExpress: tagT | |
Interactions involving this protein in SubtInteract: TagT | |
MW, pI | 35 kDa, 9.554 |
Gene length, protein length | 966 bp, 322 aa |
Immediate neighbours | ywtG, ywtE |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
cell wall synthesis, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU35840
Phenotypes of a mutant
- a tagT mutant has no phenoype, the triple tagT tagU tagV mutant is unable to grow under normal conditions PubMed
Database entries
- BsubCyc: BSU35840
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: LytR-Cps2a-Psr family PubMed
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): Mg(2+) PubMed
- Effectors of protein activity:
Database entries
- BsubCyc: BSU35840
- UniProt: Q7WY78
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: ywtF PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Stephanie Brown, John P Santa Maria, Suzanne Walker
Wall teichoic acids of gram-positive bacteria.
Annu Rev Microbiol: 2013, 67;313-36
[PubMed:24024634]
[WorldCat.org]
[DOI]
(I p)
Original publications
Alice Eberhardt, Christopher N Hoyland, Daniela Vollmer, Stephanie Bisle, Robert M Cleverley, Ola Johnsborg, Leiv S Håvarstein, Richard J Lewis, Waldemar Vollmer
Attachment of capsular polysaccharide to the cell wall in Streptococcus pneumoniae.
Microb Drug Resist: 2012, 18(3);240-55
[PubMed:22432711]
[WorldCat.org]
[DOI]
(I p)
Yoshikazu Kawai, Jon Marles-Wright, Robert M Cleverley, Robyn Emmins, Shu Ishikawa, Masayoshi Kuwano, Nadja Heinz, Nhat Khai Bui, Christopher N Hoyland, Naotake Ogasawara, Richard J Lewis, Waldemar Vollmer, Richard A Daniel, Jeff Errington
A widespread family of bacterial cell wall assembly proteins.
EMBO J: 2011, 30(24);4931-41
[PubMed:21964069]
[WorldCat.org]
[DOI]
(I e)
Judith Hübscher, Lucas Lüthy, Brigitte Berger-Bächi, Patricia Stutzmann Meier
Phylogenetic distribution and membrane topology of the LytR-CpsA-Psr protein family.
BMC Genomics: 2008, 9;617
[PubMed:19099556]
[WorldCat.org]
[DOI]
(I e)
Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862]
[WorldCat.org]
[DOI]
(I p)
Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421]
[WorldCat.org]
[DOI]
(P p)
E Presecan, I Moszer, L Boursier, H Cruz Ramos, V de la Fuente, M-F Hullo, C Lelong, S Schleich, A Sekowska, B H Song, G Villani, F Kunst, A Danchin, P Glaser
The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).
Microbiology (Reading): 1997, 143 ( Pt 10);3313-3328
[PubMed:9353933]
[WorldCat.org]
[DOI]
(P p)