yqeK

168

diphosphatase, symmetrically cleaving Ap₄A hydrolase, degradation of toxic side-products of the nicotinamide-nucleotide adenylyltransferase NadD

Locus: BSU_25630

Molecular weight: 21.14 kDa

Isoelectric point: 5.97

Protein length: 186 aa

Gene length: 561 bp

Function: degradation of Ap₄A, degradation of toxic side-products of NadD activity

Product: diphosphatase, Ap₄A hydrolase

Essential: no

Synonyms: yqeK

Genomic Context

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Categories containing this gene/protein

Metabolism, Nucleotide metabolism, Metabolism of signalling nucleotides

Metabolism, Detoxification reactions

List of homologs in different organisms, belongs to COG1713 (Galperin et al., 2021)

Gene

Coordinates: 2,643,215  2,643,775

Phenotypes of a mutant

increased levels of Ap₄A PubMed

The protein

Catalyzed reaction/ biological activity

symmetric cleavage of Ap₄A PubMed

Domains

HD domain (aa 18-132) (according to UniProt)

Structure

3CCG (PDB) (the protein from Clostridium acetobutylicum, 46% identity, 76% similarity)
P54456 (AlphaFold)

Additional information

in some organisms NadD and YqeK form one fused protein PubMed

Expression and Regulation

Operons

Genes: pgpP-yqeH-aroD-yqeI-nadD-yqeK-yqeL-yqeM

Description: PubMed

Expression

expression is increased upon depletion of depletion of tRNA maturation factors rnpB or rnz PubMed

Biological materials

Mutant

BKE25630 (yqeK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTGCTTCACGCATGCAAGTG,  downstream forward: _UP4_AGCTGAACAGGAGGAATTTG
BKK25630 (yqeK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTGCTTCACGCATGCAAGTG,  downstream forward: _UP4_AGCTGAACAGGAGGAATTTG

References

Reviews

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Original Publications

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