asnB

168

asparagine synthetase (glutamine-hydrolysing), amidates m-diaminopimelic acid

Locus: BSU_30540

Molecular weight: 72.49 kDa

Isoelectric point: 5.61

Protein length: 632 aa

Gene length: 1899 bp

Function: control of peptidoglycan hydrolysis

Product: asparagine synthetase (glutamine-hydrolysing)

Essential: yes

E.C.: 6.3.5.4

Synonyms: asnB, asn

Genomic Context

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List of homologs in different organisms, belongs to COG0367 (Galperin et al., 2021)

Gene

Coordinates: 3,125,777  3,127,675

Phenotypes of a mutant

• the mutant requires excess Mg²⁺ for growth PubMed
• the mutant id unable to grow on LB medium, but grows on minimal medium (MSgg) PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + L-aspartate + L-glutamine --> AMP + diphosphate + H⁺ + L-asparagine + L-glutamate (according to UniProt)
• amidation of meso-diaminopimelic (mDAP) acid in peptidoglycan PubMed

Protein family

• asparagine synthetase family (with AsnO and AsnH, according to UniProt)

Domains

• Glutamine amidotransferase type-2 domain (aa 2-214) (according to UniProt)

Structure

• 1CT9 (PDB) (from E. coli, 29% identity) PubMed
• P54420 (AlphaFold)

Expression and Regulation

Operons

• Genes: metK-asnB-alaP

  Description: PubMed

  Regulation

  • constitutive

Biological materials

Mutant

• MGNA-A141 (asnB::erm), available at the NBRP B. subtilis, Japan

References

Reviews

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Original Publications

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