Difference between revisions of "EfeU"

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* '''Description:''' elemental iron uptake system (permease) <br/><br/>
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* '''Description:''' elemental iron uptake system (permease), high affinity uptake of ferric iron (Fe(III)) <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Product''' || elemental iron uptake system (permease)  
 
|style="background:#ABCDEF;" align="center"| '''Product''' || elemental iron uptake system (permease)  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || iron uptake
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|style="background:#ABCDEF;" align="center"|'''Function''' || elemental iron uptake
 +
|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU38280 efeU]
 +
|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/EfeU EfeU]
 +
|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in  [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=EfeU EfeU]'''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 52 kDa, 8.958   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 52 kDa, 8.958   
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1443 bp, 481 aa  
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1443 bp, 481 aa  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywbM]]'', ''[[thiE]]''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[efeO]]'', ''[[thiE]]''
 
|-
 
|-
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15854&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU38280 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU38280 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU38280 DNA_with_flanks]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ywbL_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ywbL_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 +
|-
 +
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=efeU_3929105_3930550_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:ywbL_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU38280]]
 
|-
 
|-
 
|}
 
|}
  
 
__TOC__
 
__TOC__
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/>
 +
 +
= [[Categories]] containing this gene/protein =
 +
{{SubtiWiki category|[[transporters/ other]]}},
 +
{{SubtiWiki category|[[acquisition of iron]]}},
 +
{{SubtiWiki category|[[iron metabolism]]}},
 +
{{SubtiWiki category|[[membrane proteins]]}}
  
−
<br/><br/>
+
= This gene is a member of the following [[regulons]] =
 +
{{SubtiWiki regulon|[[Fur regulon]]}}
  
 
=The gene=
 
=The gene=
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=== Additional information===
 
=== Additional information===
 +
 +
  
  
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
−
* '''Interactions:'''
+
* '''[[SubtInteract|Interactions]]:'''
 +
** [[EfeO]]-[[EfeU]]-[[EfeB]] {{PubMed|23180473,23764491}}
  
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* '''Localization:''' cell membrane (according to Swiss-Prot)
+
* '''[[Localization]]:'''  
 +
** cell membrane (according to Swiss-Prot)
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Structure:'''
 
* '''Structure:'''
  
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* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P39595 P39595]
+
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39595 P39595]
  
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU38280]
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU38280]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
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=Expression and regulation=
 
=Expression and regulation=
 +
* '''Operon:'''
 +
** ''[[efeU]]-[[efeO]]-[[efeB]]'' {{PubMed|9353933}}
  
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* '''Operon:'''  
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=efeU_3929105_3930550_-1 efeU] {{PubMed|22383849}}
  
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* '''[[Sigma factor]]:'''  
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* '''Sigma factor:''' [[SigA]] {{PubMed|23764491}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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** repressed unless the cells enter an iron starvation ([[Fur]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12354229 PubMed]
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** induced upon iron starvation ([[Fur]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/12354229 PubMed]
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 
** [[Fur]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12354229 PubMed]
 
** [[Fur]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12354229 PubMed]
  
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* '''Additional information:'''  
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* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
  
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<pubmed>16672620,,12354229, </pubmed>
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<pubmed>16672620,23180473,12354229, 23764491</pubmed>
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 +
[[Category:Protein-coding genes]]

Latest revision as of 11:45, 8 April 2014

  • Description: elemental iron uptake system (permease), high affinity uptake of ferric iron (Fe(III))

Gene name ywbL
Synonyms ipa-27d
Essential no
Product elemental iron uptake system (permease)
Function elemental iron uptake
Gene expression levels in SubtiExpress: efeU
Interactions involving this protein in SubtInteract: EfeU
Metabolic function and regulation of this protein in SubtiPathways:
EfeU
MW, pI 52 kDa, 8.958
Gene length, protein length 1443 bp, 481 aa
Immediate neighbours efeO, thiE
Sequences Protein DNA DNA_with_flanks
Genetic context
YwbL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YwbL expression.png















Categories containing this gene/protein

transporters/ other, acquisition of iron, iron metabolism, membrane proteins

This gene is a member of the following regulons

Fur regulon

The gene

Basic information

  • Locus tag: BSU38280

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Marcus Miethke, Carmine G Monteferrante, Mohamed A Marahiel, Jan Maarten van Dijl
The Bacillus subtilis EfeUOB transporter is essential for high-affinity acquisition of ferrous and ferric iron.
Biochim Biophys Acta: 2013, 1833(10);2267-78
[PubMed:23764491] [WorldCat.org] [DOI] (P p)

Carmine G Monteferrante, Calum MacKichan, Elodie Marchadier, Maria-Victoria Prejean, Rut Carballido-López, Jan Maarten van Dijl
Mapping the twin-arginine protein translocation network of Bacillus subtilis.
Proteomics: 2013, 13(5);800-11
[PubMed:23180473] [WorldCat.org] [DOI] (I p)

Juliane Ollinger, Kyung-Bok Song, Haike Antelmann, Michael Hecker, John D Helmann
Role of the Fur regulon in iron transport in Bacillus subtilis.
J Bacteriol: 2006, 188(10);3664-73
[PubMed:16672620] [WorldCat.org] [DOI] (P p)

Noel Baichoo, Tao Wang, Rick Ye, John D Helmann
Global analysis of the Bacillus subtilis Fur regulon and the iron starvation stimulon.
Mol Microbiol: 2002, 45(6);1613-29
[PubMed:12354229] [WorldCat.org] [DOI] (P p)

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