artQ

168

high affinity [metabolite|arginine] [wiki|ABC transporter] (permease)

locus: BSU_23970

Molecular weight: 23.75 kDa

pI: 9.08

Protein length: 219 aa

Gene length: 660 bp

function: [metabolite|arginine] uptake

product: high affinity [metabolite|arginine] [wiki|ABC transporter] (permease)

essential: no

synonyms: artQ, yqiY

List of homologs in different organisms, belongs to COG0765 (Galperin et al., 2021)

Gene

Coordinates: 2,491,289  2,491,948

The protein

Catalyzed reaction/ biological activity

• transport of [metabolite|arginine]

Protein family

• [wiki|Binding-protein-dependent transport system permease family] (according to UniProt)

[wiki|Domains]

• [wiki|ABC transmembrane type-1 domain] (aa 19-208) (according to UniProt)

Structure

• [PDB|4YMS] (from Caldanaerobacter subterraneus, 44% identity) [pubmed|25848002]
• [AF|P54536]

[wiki|Localization]

• cell membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|8D9529D46E11D8B2826A98642706ADCBE1E3434A|artP]-[gene|62BD291B91DB0358754957D84A1F3636C247A697|artQ]-[gene|6882EF93EC82872B25C88104F62D6603D2514890|artR]

  description: [Pubmed|12107147]

  regulation

  • repressed by casamino acids [Pubmed|12107147]
  • induced in the absence of [protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM] [pubmed|30355672]

  regulatory mechanism

  • [protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM]: unknown, [pubmed|30355672], in [regulon|protein:F4097349A563503468A2A14F062AEAC532C7917A|rnpM regulon]

Biological materials

Mutant

• GP1689 Δ([gene|8D9529D46E11D8B2826A98642706ADCBE1E3434A|artP]-[gene|62BD291B91DB0358754957D84A1F3636C247A697|artQ]-[gene|6882EF93EC82872B25C88104F62D6603D2514890|artR])::''mls'', available in [wiki|Jörg Stülke]'s lab
• GP4156 (Δ[gene|9BFB16C12B31ABD70BBB921BCB9E4201E3BDC92D|rocC]::''aphA3'', Δ[gene|8D9529D46E11D8B2826A98642706ADCBE1E3434A|artP]-[gene|62BD291B91DB0358754957D84A1F3636C247A697|artQ]-[gene|6882EF93EC82872B25C88104F62D6603D2514890|artR]::''ermC'', Δ[gene|B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6|rocE]::''tet'',), available in [wiki|Jörg Stülke]'s lab
• MGNA-C382 ([gene|62BD291B91DB0358754957D84A1F3636C247A697|artQ]::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2380 NBRP B. subtilis, Japan]
• BKE23970 ([gene|62BD291B91DB0358754957D84A1F3636C247A697|artQ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGCCTTTGTCTCCTTTT,  downstream forward: _UP4_GCTGTAGAAAGGAAGCTGAA
• BKK23970 ([gene|62BD291B91DB0358754957D84A1F3636C247A697|artQ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGCCTTTGTCTCCTTTT,  downstream forward: _UP4_GCTGTAGAAAGGAAGCTGAA

References

• 10092453,12107147,18763711,11423008,25848002,39397791