murC

168

UDP-N-acetylmuramoyl-L-alanine synthetase

Locus: BSU_29790

Molecular weight: 48.20 kDa

Isoelectric point: 5.31

Protein length: 432 aa

Gene length: 1299 bp

Function: peptidoglycan precursor biosynthesis

Product: UDP-N-acetylmuramoyl-L-alanine synthetase

Essential: yes

E.C.: 6.3.2.8

Synonyms: murC, ytxF

Genomic Context

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List of homologs in different organisms, belongs to COG0773 (Galperin et al., 2021)

Gene

Coordinates: 3,048,177  3,049,475

Phenotypes of a mutant

• essential PubMed
• murC is dispensable in wall-less L-forms PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + L-alanine + UDP-N-acetyl-α-D-muramate --> ADP + H⁺ + phosphate + UDP-N-acetyl-α-D-muramoyl-L-alanine (according to UniProt)

Protein family

• MurCDEF family (according to UniProt)

Structure

• 1GQQ (PDB) (from Haemophilus influenzae, 31% identity)
• P40778 (AlphaFold)

Localization

• cytoplasm (Homogeneous) PubMed
• forms banded patterns along the length of the cell, this pattern formation depends on MreB PubMed

Expression and Regulation

Operons

• Genes: murC

  Description: PubMed

• Genes: sftA-murC

  Description: PubMed

Biological materials

Expression vectors

• pGP3746 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in Jörg Stülke's lab

FLAG-tag construct

• GP4406 murC-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab

LacZ fusion

• pGP3743 (in pAC5), available in Jrög Stülke's lab

References

Reviews

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Original Publications

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