cimH

168

transporter for [metabolite|citrate] (proton symport)

locus: BSU_38770

Molecular weight: 48.18 kDa

pI: 6.55

Protein length: 450 aa

Gene length: 1353 bp

function: [metabolite|citrate] uptake

product: transporter for [metabolite|citrate] (proton symport)

essential: no

synonyms: cimH, yxkJ

List of homologs in different organisms, belongs to COG3493 (Galperin et al., 2021)

Gene

Coordinates: 3,979,753 → 3,981,105

The protein

Catalyzed reaction/ biological activity

• uptake of [metabolite|citrate] (symport with protons) under conditions of [metabolite|glutamate] deficiency [Pubmed|40490120,11566984]

Protein family

• sodium:citrate (SCF) symporter family (together with [protein|F8F62635AA87E4E3A255D6F8FC621EF40954B520|maeN], according to UniProt)

Structure

• [PDB|5X9R] (from Klebsiella pneumoniae, 34% identity) [pubmed|28566738]
• [AF|P94363]

Paralogous protein(s)

• [protein|F8F62635AA87E4E3A255D6F8FC621EF40954B520|maeN]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|FEC0EDE9663FE62B2A1A4FA5B16B41048DDD723B|cimH]

  description: [Pubmed|21815947]

  regulation

  • repressed by glucose ([protein|search|CcpA]) [Pubmed|10666464]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|10666464], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]

  additional information

  • the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• MGNA-B744 (yxkJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1743 NBRP B. subtilis, Japan]
• BKE38770 (Δ[gene|FEC0EDE9663FE62B2A1A4FA5B16B41048DDD723B|cimH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38770 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGAAAAACCTCCCCTAT,  downstream forward: _UP4_TGATCCCCGCAAGCAAGTTC
• BKK38770 (Δ[gene|FEC0EDE9663FE62B2A1A4FA5B16B41048DDD723B|cimH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38770 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGAAAAACCTCCCCTAT,  downstream forward: _UP4_TGATCCCCGCAAGCAAGTTC
• GP4216 ([gene|FEC0EDE9663FE62B2A1A4FA5B16B41048DDD723B|cimH]::kan), available in Jörg Stülke's lab

References

Reviews

• 16339740

Original Publications

• 12525174,10666464,11566984,21815947,28566738,40490120