skfC

168

may be involved in spore killing

locus: BSU_01935

Molecular weight: 56.00 kDa

pI: 8

Protein length: 496 aa

Gene length: 1491 bp

function: may be involved in spore killing

product: unknown

essential: no

synonyms: skfC, ybcS

List of homologs in different organisms

Gene

Coordinates: 215,404  216,894

Expression and Regulation

Operons

• genes: [gene|DDB0022F50999AC52809D651C9CC5A5FDC71302C|skfA]-[gene|F981C605C652D1A4429579A5F24608CE7F570834|skfB]-[gene|4DFF9924E07D834F37580D73E88451C1671C5111|skfC]-[gene|5886364199844356446C08F08664B301A714E74A|skfE]-[gene|77D93CAEF46EBBB9BAF570714445E194D5F2884E|skfF]-[gene|527AB8EAAF0A1AAD4E5B714B35ED9F78BECC17EC|skfG]-[gene|F31B12F80AFC3580FD33811A1C22E74F75DC1A85|skfH]

  description: [Pubmed|16816204]

  regulation

  • expressed under conditions that trigger sporulation ([wiki|Spo0A]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647,15687200 PubMed]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: activation, [PubMed|14651647,15687200], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [PubMed|15687200,17720793], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: activation, [Pubmed|16816204], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16452424], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

  additional information

  • Northern blotting during during phosphate limitation showed an intense 0.25 kb '[protein|search|skfA]'-specific transcript, and a weaker 6.5 kb '[protein|search|skfA]-[protein|search|skfB]-[protein|search|skfC]-[protein|search|skfE]-[protein|search|skfF]-[protein|search|skfG]-[protein|search|skfH]' transcript.

Biological materials

Mutant

• MGNA-C514 (ybcS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2512 NBRP B. subtilis, Japan]
• BKE01935 ([gene|4DFF9924E07D834F37580D73E88451C1671C5111|skfC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01935 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CCAGAACACCAATGATAGAC,  downstream forward: _UP4_CTCTAATGATTAGGAGAAGA
• BKK01935 ([gene|4DFF9924E07D834F37580D73E88451C1671C5111|skfC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01935 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CCAGAACACCAATGATAGAC,  downstream forward: _UP4_CTCTAATGATTAGGAGAAGA

References

Reviews

• 20955377

Original Publications

• 12817086,15812018,16816204,14651647,15687200,15687200,17720793,17449691,18840696,27766092