yknX

168

ABC-type antimicrobial peptide transporter (permease) for the export of the SdpC toxin

Locus: BSU_14350

Molecular weight: 41.55 kDa

Isoelectric point: 5.18

Protein length: 377 aa

Gene length: 1134 bp

Function: resistence against SdpC toxin

Product: ABC-type antimicrobial peptide exporter (permease)

Essential: no

Synonyms: yknX

Genomic Context

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List of homologs in different organisms, belongs to COG0845 (Galperin et al., 2021)

Gene

Coordinates: 1,504,282 → 1,505,415

The protein

Protein family

• Membrane fusion protein (MFP) (TC 8.A.1) family (according to UniProt)

Structure

• 4DK0 (PDB) (from Actinobacillus actinomycetemcomitans, 24% identity) PubMed
• O31710 (AlphaFold)

Localization

• membrane protein with a large extracytoplasmic domain  PubMed

Expression and Regulation

Operons

• Genes: yknU-yknV-yknW-yknX-yknY-yknZ

  Description: PubMed

• Genes: yknW-yknX-yknY-yknZ

  Description: PubMed

  Regulation

  • repressed during logrithmic growth (AbrB) PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon

  Sigma factors

  • SigW: sigma factor, PubMed, in sigW regulon

Biological materials

Mutant

• MGNA-B349 (yknX::erm), available at the NBRP B. subtilis, Japan
• BKE14350 (ΔyknX::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TCCGATCCAGACTTTTTTCA,  downstream forward: _UP4_GACGGAATGGAAGTGAAATC
• BKK14350 (ΔyknX::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TCCGATCCAGACTTTTTTCA,  downstream forward: _UP4_GACGGAATGGAAGTGAAATC

References

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