dppE

168

dipeptide ABC transporter (dipeptide-binding protein)

Locus: BSU_12960

Molecular weight: 62.41 kDa

Isoelectric point: 5.52

Protein length: 549 aa

Gene length: 1650 bp

Function: uptake of dipeptides

Product: dipeptide ABC transporter (dipeptide-binding protein)

Essential: no

Synonyms: dppE, dciAE

Genomic Context

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List of homologs in different organisms, belongs to COG4166 (Galperin et al., 2021)

Gene

Coordinates: 1,364,151  1,365,800

The protein

Protein family

• bacterial solute-binding protein 5 family (according to UniProt)

Structure

• 8AZB (PDB) PubMed
• 4FAJ (PDB) (from Enterococcus faecalis, 33% identity) PubMed
• P26906 (AlphaFold)

Paralogous protein(s)

• OppA

Localization

• attached to the cell membrane (via DppB-DppC) PubMed

Expression and Regulation

Operons

• Genes: dppA-dppB-dppC-dppD-dppE

  Description: PubMed

  Regulation

  • repressed by glucose (2.9-fold) PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, in codY regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: dppA-dppB-dppC-dppD-dppE-ykfA-ykfB-ykfC-ykfD

  Description: PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, in codY regulon

Biological materials

Mutant

• GP1110 (spc), available in Jörg Stülke's lab
• 1A737 ( dppE::kan), PubMed, available at BGSC
• BKE12960 (dppE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCTCTTCCCCCTTTTCA,  downstream forward: _UP4_TGATGGAGGCGATTGAGGAA
• BKK12960 (dppE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCTCTTCCCCCTTTTCA,  downstream forward: _UP4_TGATGGAGGCGATTGAGGAA

References

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