purC

168

phosphoribosylaminoimidazole succinocarboxamide synthase

Locus: BSU_06450

Molecular weight: 27.31 kDa

Isoelectric point: 4.88

Protein length: 241 aa

Gene length: 726 bp

Function: purine biosynthesis

Product: phosphoribosylaminoimidazole succinocarboxamide synthase

Essential: no

E.C.: 6.3.2.6

Synonyms: purC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0152 (Galperin et al., 2021)

Gene

Coordinates: 701,601 702,326

The protein

Catalyzed reaction/ biological activity

• 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP + L-aspartate --> (2S)-2-[5-amino-1-(5-phospho--D-ribosyl)imidazole-4-carboxamido]succinate + ADP + 2 H⁺ + phosphate (according to UniProt)

Protein family

• SAICAR synthetase family (according to Swiss-Prot)

Structure

• 1KUT (PDB) (from Thermotoga maritima, 40% identity, 57% similarity) PubMed
• P12046 (AlphaFold)

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: purE-purK-purB-purC-purS-purQ-purL-purF-purM-purN-purH-purD

  Description: PubMed

  Regulation

  • expression activated by glucose (4.4 fold) PubMed

  Regulatory mechanism

  • G-box: RNA switch, (riboswitch) PubMed, in G-box
  • PurR: repression, (molecular inducer: PRPP) PubMed, in purR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE06450 (purC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGGAAGGCCTCCTAACCC, downstream forward: _UP4_TTCAATAGACTGGGAGGCAT
• BKK06450 (purC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGGAAGGCCTCCTAACCC, downstream forward: _UP4_TTCAATAGACTGGGAGGCAT

References

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