katX

168

catalase, general stress protein

locus: BSU_38630

Molecular weight: 62.19 kDa

pI: 5.34

Protein length: 547 aa

Gene length: 1644 bp

function: detoxification (degradation) of hydrogen peroxide

product: catalase (major catalase in spores)

essential: no

synonyms: katX, yxlI

List of homologs in different organisms, belongs to COG0753 (Galperin et al., 2021)

Gene

Coordinates: 3,964,997 → 3,966,640

The protein

Catalyzed reaction/ biological activity

• 2 H₂O₂ --> 2 H₂O + O₂ (according to UniProt)

Protein family

• [wiki|catalase family] (according to UniProt)

Structure

• [PDB|4CAB] (from Deinococcus radiodurans, 69% identity) [pubmed|24975828]
• [AF|P94377]

Paralogous protein(s)

• [protein|B0A1AB6E920E6CBEE45115297599AF9A80972E38|katA], [protein|03FE63D6AAC77D6CEB052D0BF578ACD048C772FB|katE]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|DA5EB526EE8AF41BE9B837F2C276432BC4183AA1|katX]

  description: [Pubmed|10503549]

  regulation

  • expressed early during sporulation in the forespore ([protein|search|SigF], [protein|search|RsfA]) [Pubmed|16497325,15699190,10629188]

  regulatory mechanism

  • [protein|7C0458DB0E3E3AC952FB9DDBC4AD220A3A456359|rsfA]: activation, [Pubmed|10629188], in [regulon|protein:7C0458DB0E3E3AC952FB9DDBC4AD220A3A456359|rsfA regulon]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|10503549,15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|10503549,16497325], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]

Biological materials

Mutant

• MGNA-B755 (katX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1754 NBRP B. subtilis, Japan]
• BKE38630 (Δ[gene|DA5EB526EE8AF41BE9B837F2C276432BC4183AA1|katX]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38630 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGGTTGGCCCTCCTTTT,  downstream forward: _UP4_TAACAACAGCGAACAGGGCT
• BKK38630 (Δ[gene|DA5EB526EE8AF41BE9B837F2C276432BC4183AA1|katX]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38630 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATGGTTGGCCCTCCTTTT,  downstream forward: _UP4_TAACAACAGCGAACAGGGCT

References

• 9393707,10503549,16497325,9555886,15805528,10629188,24975828