msrB

168

peptide methionine sulfoxide reductase

Locus: BSU_21680

Molecular weight: 16.46 kDa

Isoelectric point: 5.84

Protein length: 143 aa

Gene length: 432 bp

Function: regeneration of methionine and restoration of protein function after oxidative damage

Product: peptide methionine sulfoxide reductase

E.C.: 1.8.4.12

Synonyms: msrB, yppQ

Genomic Context

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List of homologs in different organisms, belongs to COG0229 (Galperin et al., 2021)

Gene

Coordinates: 2,287,097 → 2,287,528

The protein

Catalyzed reaction/ biological activity

• [thioredoxin]-disulfide + H₂O + L-methionyl-[protein] --> [thioredoxin]-dithiol + L-methionyl-(R)-S-oxide-[protein] (according to UniProt)

Protein family

• MsrB Met sulfoxide reductase family (single member, according to UniProt)

Domains

• MsrB domain (aa 5-126) (according to UniProt)

Structure

• 1XM0 (PDB)
• P54155 (AlphaFold)

Expression and Regulation

Operons

• Genes: msrA-msrB

  Description: PubMed

  Regulation

  • induced by oxidative stress (Spx)

  Regulatory mechanism

  • Spx: activation, oxidized Spx binds RpoA and enhances MsrA-MsrB operon transcription PubMed, in spx regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A905 (yppQ::erm), available at the NBRP B. subtilis, Japan
• BKE21680 (ΔmsrB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTTTCTTCTTTATTGTACG,  downstream forward: _UP4_TAAACTGTTAAAAACAGGCC
• BKK21680 (ΔmsrB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTTTCTTCTTTATTGTACG,  downstream forward: _UP4_TAAACTGTTAAAAACAGGCC

References

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