cdaR
168
sensor of cell wall integrity, stimulates CdaA diadenylate cyclase activity in the presence of cell wall defects
Locus
BSU_01760
Molecular weight
52.78 kDa
Isoelectric point
4.72
Function
regulation of c-di-AMP synthesis
Product
effector protein controlling CdaA diadenylate cyclase activity
Essential
no
Synonyms
cdaR, ybbR
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4856 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
197,027 → 198,478
The protein
Catalyzed reaction/ biological activity
senses cell wall defects PubMed
YbbR-like 1 domain (aa 55-135) (according to UniProt)
YbbR-like 2 domain (aa 143-228) (according to UniProt)
YbbR-like 3 domain (aa 237-316) (according to UniProt)
YbbR-like 4 domain (aa 329-394) (according to UniProt)
C-terminal intrinsically disordered domain PubMed
Structure
2KPU (PDB) (one YbbR domain, from Desulfitobacterium hafniense) PubMed
cell membrane, oriented towards the outside PubMed
Expression and Regulation
Operons
Description
Regulatory mechanism
GlmS ribozyme: RNA switch, in GlmS ribozyme
Sigma factors
Open in new tab
Additional information
the mRNA is very stable (> 15 min) PubMed
Biological materials
Mutant
Expression vectors
IPTG inducible expression of Strep-cdaR in E. coli: pGP2565 (in pGP172), available in Jörg Stülke's lab
IPTG inducible expression of His-cdaR in E. coli: pGP2557 (in pET19b), available in Jörg Stülke's lab
GP2031: cdaR-Strep aphA3, chromosomal expression in B. subtilis, for SPINE, available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab. Respective plasmids: pGP1989 and pGP1992 PubMed
Antibody
available in Jörg Stülke's lab
References
Reviews
Loading
Original Publications
Loading
Page visits: 6435
Time of last update: 2025-06-21 01:24:39
Author of last update: Jstuelk