cdaR

168

effector protein controlling [protein|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA] diadenylate cyclase activity

locus: BSU_01760

Molecular weight: 52.78 kDa

pI: 4.72

Protein length: 483 aa

Gene length: 1452 bp

function: regulation of c-di-AMP synthesis

product: effector protein controlling [protein|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA] diadenylate cyclase activity

essential: no

synonyms: cdaR, ybbR

List of homologs in different organisms, belongs to COG4856 (Galperin et al., 2021)

Gene

Coordinates: 197,027 → 198,478

The protein

Catalyzed reaction/ biological activity

• stimulates [protein|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA] activity upon protein-protein interaction [Pubmed|23192352]

[wiki|Domains]

• YbbR-like 1 domain (aa 55-135) (according to UniProt)
• YbbR-like 2 domain (aa 143-228) (according to UniProt)
• YbbR-like 3 domain (aa 237-316) (according to UniProt)
• YbbR-like 4 domain (aa 329-394) (according to UniProt)

Structure

• [PDB|2KPU] (one YbbR domain, from ''Desulfitobacterium hafniense'') [Pubmed|21154411]
• [AF|O34659]

[wiki|Localization]

• cell membrane, oriented towards the outside [Pubmed|26240071]

Expression and Regulation

Operons

• genes: [gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA]-[gene|FB23F2AB0E2A82658009D115E5876E7AF1BDE5FD|cdaR]-[gene|F022ACB2CBB8DA46B392CE1D26474093645F26DA|glmM]-[gene|4E23D2043D51C587027ABBF6335F148295E90B8F|glmS]

  description: [Pubmed|23192352]

  regulatory mechanism

  • GlmS ribozyme: RNA switch, in [regulon|other_regulator:GlmS ribozyme|GlmS ribozyme]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|22211522], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

additional information

• the mRNA is very stable (> 15 min) [pubmed|12884008]

Biological materials

Mutant

• GP999 (Δ[gene|FB23F2AB0E2A82658009D115E5876E7AF1BDE5FD|cdaR]::''cat''), available in [wiki|Jörg Stülke]'s lab [pubmed|23192352]
• GP985 Δ[gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA]-[gene|FB23F2AB0E2A82658009D115E5876E7AF1BDE5FD|cdaR]::''cat''), available in [wiki|Jörg Stülke]'s lab [pubmed|23192352]
• BKE01760 (Δ[gene|FB23F2AB0E2A82658009D115E5876E7AF1BDE5FD|cdaR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01760 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAGCCCAGCGGTTGTTTA,  downstream forward: _UP4_GAATAAAAAAGGAGCGATTA
• BKK01760 (Δ[gene|FB23F2AB0E2A82658009D115E5876E7AF1BDE5FD|cdaR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01760 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAGCCCAGCGGTTGTTTA,  downstream forward: _UP4_GAATAAAAAAGGAGCGATTA

Expression vectors

• IPTG inducible expression of Strep-''cdaR'' in ''E. coli'': pGP2565 (in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
• IPTG inducible expression of His-''cdaR'' in ''E. coli'': pGP2557 (in [http://biochem.web.utah.edu/hill/links/pET19b.pdf pET19b]), available in [wiki|Jörg Stülke]'s lab
• GP2031: ''cdaR-Strep aphA3'', chromosomal expression in B. subtilis, for [protein|search|SPINE], available in [wiki|Jörg Stülke]'s lab

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab. Respective plasmids: pGP1989 and pGP1992 [pubmed|23192352]

Antibody

• available in [wiki|Jörg Stülke]'s lab

References

Reviews

• 25869574,30224435,32603625

Original Publications

• 12884008,16236721,26240071,23192352,22211522,21154411,26025048,26527648,27834680,32250026