citH

168

sporulation-specific secondary transporter of divalent metal ions/citrate complexes

Locus: BSU_39060

Molecular weight: 45.37 kDa

Isoelectric point: 9.28

Protein length: 426 aa

Gene length: 1281 bp

Function: citrate uptake

Product: secondary transporter of divalent metal ions/citrate complexes

Essential: no

Synonyms: citH, yxiQ

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2851 (Galperin et al., 2021)

Gene

Coordinates: 4,010,404  4,011,684

The protein

Protein family

• CitM transporter family (TC 2.A.11) (according to UniProt)

Structure

• P42308 (AlphaFold)

Paralogous protein(s)

• CitM

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: citH

  Description: PubMed

  Regulation

  • expressed during sporulation PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon
  • SigF: sigma factor, in sigF regulon

Biological materials

Mutant

• MGNA-B723 (yxiQ::erm), available at the NBRP B. subtilis, Japan
• BKE39060 (citH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATATGACCTCCCCTAT,  downstream forward: _UP4_TAAATGTATGAGCTTGATCG
• BKK39060 (citH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATATGACCTCCCCTAT,  downstream forward: _UP4_TAAATGTATGAGCTTGATCG

References

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