Difference between revisions of "IseA"

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* '''Description:''' inhibits in vitro activity of cell wall endopeptidases LytE and LytF, inhibits cell separation <br/><br/>
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* '''Description:''' inhibits in vitro activity of cell wall endopeptidases [[LytE]] and [[LytF]], inhibits cell separation <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || protection against cell envelope stress
 
|style="background:#ABCDEF;" align="center"|'''Function''' || protection against cell envelope stress
 +
|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU18380 iseA]
 +
|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=IseA IseA]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 10.176   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 10.176   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yoeA]]'', ''[[trnSL-Arg1]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yoeA]]'', ''[[trnSL-Arg1]]''
 
|-
 
|-
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13721&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU18380 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU18380 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU18380 DNA_with_flanks]
 
|-
 
|-
 
|-
 
|-
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|colspan="2" | '''Genetic context''' <br/> [[Image:yoeB_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yoeB_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|-
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=iseA_2002637_2003182_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:iseA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU18380]]
 
|-
 
|-
 
|}
 
|}
  
 
__TOC__
 
__TOC__
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/>
  
−
<br/><br/>
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= [[Categories]] containing this gene/protein =
 +
{{SubtiWiki category|[[cell wall degradation/ turnover]]}},
 +
{{SubtiWiki category|[[membrane proteins]]}}
 +
 
 +
= This gene is a member of the following [[regulons]] =
 +
{{SubtiWiki regulon|[[WalR regulon]]}}
  
 
=The gene=
 
=The gene=
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU18380&redirect=T BSU18380]
  
 
* '''DBTBS entry:''' no entry
 
* '''DBTBS entry:''' no entry
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=== Additional information===
 
=== Additional information===
 +
 +
  
  
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
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* '''Interactions:''' [[IseA]]-[[LytF]] (catalytic domain)
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* '''[[SubtInteract|Interactions]]:'''
 +
** [[IseA]]-[[LytF]] (catalytic domain) {{PubMed|18761694}}
  
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* '''Localization:''' cell membrane (according to Swiss-Prot)
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* '''[[Localization]]:''' cell membrane (according to Swiss-Prot), localized to cell separation sites
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU18380&redirect=T BSU18380]
  
 
* '''Structure:'''
 
* '''Structure:'''
  
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* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O34841 O34841]
+
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34841 O34841]
  
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU18380]
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU18380]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
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=Expression and regulation=
 
=Expression and regulation=
  
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* '''Operon:'''  
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* '''Operon:''' ''iseA'' {{PubMed|22383849}}
 +
 
 +
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=iseA_2002637_2003182_1 iseA] {{PubMed|22383849}}
  
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* '''[[Sigma factor]]:'''  
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* '''Sigma factor:'''  
  
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* '''Regulation:''' repessed by [[WalR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17581128 PubMed]
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* '''Regulation:'''  
 +
** repressed by [[WalR]] {{PubMed|17581128}}
 +
** induced by vancomycin {{PubMed|17483219}}
 +
** strongly induced in response to glucose starvation in M9 medium {{PubMed|23033921}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 +
** [[WalR]]: transcription repression  {{PubMed|17581128}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium): 188 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
 +
<pubmed>17581128,18761694,17483219, 20059685,22383849 23091053,23033921 23199363</pubmed>
  
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# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
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[[Category:Protein-coding genes]]

Latest revision as of 09:59, 17 April 2014

  • Description: inhibits in vitro activity of cell wall endopeptidases LytE and LytF, inhibits cell separation

Gene name iseA
Synonyms yoeB
Essential no
Product inhibitor of autolysins
Function protection against cell envelope stress
Gene expression levels in SubtiExpress: iseA
Interactions involving this protein in SubtInteract: IseA
MW, pI 19 kDa, 10.176
Gene length, protein length 543 bp, 181 aa
Immediate neighbours yoeA, trnSL-Arg1
Sequences Protein DNA DNA_with_flanks
Genetic context
YoeB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
IseA expression.png















Categories containing this gene/protein

cell wall degradation/ turnover, membrane proteins

This gene is a member of the following regulons

WalR regulon

The gene

Basic information

  • Locus tag: BSU18380

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cell membrane (according to Swiss-Prot), localized to cell separation sites

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed by WalR PubMed
    • induced by vancomycin PubMed
    • strongly induced in response to glucose starvation in M9 medium PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 188 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

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