thrZ

168

threonyl-tRNA synthetase (minor)

Locus: BSU_37560

Molecular weight: 73.20 kDa

Isoelectric point: 5.75

Protein length: 638 aa

Gene length: 1917 bp

Function: translation

Product: threonyl-tRNA synthetase (minor)

Essential: no

E.C.: 6.1.1.3

Synonyms: thrZ, thrS2

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0441 (Galperin et al., 2021)

Gene

Coordinates: 3,854,256 → 3,856,172

The protein

Catalyzed reaction/ biological activity

• ATP + L-threonine + tRNA^Thr --> AMP + diphosphate + H⁺ + L-threonyl-tRNA^Thr (according to UniProt)

Protein family

• Class-II aminoacyl-tRNA synthetase family (according to UniProt)

Domains

• TGS domain (aa 1-64) (according to UniProt)

Structure

• 1TJE (PDB) (from Escherichia coli, 43% identity, 65% similarity) PubMed
• P18256 (AlphaFold)

Paralogous protein(s)

• ThrS, one of the two proteins has to be present for viability PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and Regulation

Operons

• Genes: thrZ-ywhA

  Description: PubMed

  Regulation

  • induced by threonine limitation (T-box) PubMed
  • the T-box RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • T-box: RNA switch, in T-box

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE37560 (ΔthrZ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTAGAACACTCCTTAT,  downstream forward: _UP4_TAGGATAAAAGCTGTGAATT
• BKK37560 (ΔthrZ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTAGAACACTCCTTAT,  downstream forward: _UP4_TAGGATAAAAGCTGTGAATT

References

Loading