malP

168

maltose-specific permease of the phosphotransferase system, EIICB of the PTS

Locus: BSU_08200

Molecular weight: 57.89 kDa

Isoelectric point: 8.87

Protein length: 527 aa

Gene length: 1584 bp

Function: maltose uptake and phosphorylation

Product: maltose-specific PTS, EIICB

Essential: no

E.C.: 2.7.1.69

Synonyms: malP, glvC

Genomic Context

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List of homologs in different organisms, belongs to COG1264 (Galperin et al., 2021)

Gene

Coordinates: 892,215 → 893,798

Phenotypes of a mutant

• no growth on maltose as single carbon source PubMed

The protein

Catalyzed reaction/ biological activity

• D-maltose + N^π-phospho-L-histidyl-[protein] --> α-maltose 6'-phosphate + L-histidyl-[protein] (according to UniProt)

Protein family

• PTS permease, glucose family PubMed

Domains

• PTS EIIB domain type-1 (aa 449–527) (according to UniProt)
• PTS EIIC domain type-1 (aa 1-418) (according to UniProt)

Structure

• 6BVG (PDB) (EIIC domain from B. cereus, corresponds to aa 6 - 419, 27% identity) PubMed
• P54715 (AlphaFold)

Paralogous protein(s)

• NagP, GamP, PtsG

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: malA-glvR-malP

  Description: PubMed

  Regulation

  • induction by maltose (GlvR) PubMed

  Regulatory mechanism

  • GlvR: activation, PubMed, in glvR regulon
  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-C291 (glvC::erm), available at the NBRP B. subtilis, Japan
• GP110 (spc), available in Stülke lab
• BKE08200 (ΔmalP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGCATCATACCAGAACCCC,  downstream forward: _UP4_TAAAGACTGCCCTCCTTTTC
• BKK08200 (ΔmalP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGCATCATACCAGAACCCC,  downstream forward: _UP4_TAAAGACTGCCCTCCTTTTC

References

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