trpE

168

anthranilate synthase (subunit I)

Locus: BSU_22680

Molecular weight: 57.95 kDa

Isoelectric point: 5.25

Protein length: 515 aa

Gene length: 1548 bp

Function: biosynthesis of tryptophan

Product: anthranilate synthase (subunit I)

Essential: no

E.C.: 4.1.3.27

Synonyms: trpE

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0147 (Galperin et al., 2021)

Gene

Coordinates: 2,375,869  2,377,416

The protein

Catalyzed reaction/ biological activity

• chorismate + L-glutamine --> anthranilate + H⁺ + L-glutamate + pyruvate (according to UniProt)

Protein family

• Anthranilate synthase component I family (with PabB, according to UniProt)

Structure

• 1I7Q (PDB) (from Serratia marcescens, 42% identity, 62% similarity) PubMed
• P03963 (AlphaFold)

Effectors of protein activity

• subject to feedback inhibtion by tryptophan PubMed

Expression and Regulation

Operons

• Genes: trpE-trpD-trpC-trpF-trpB-trpA

  Description: PubMed

  Regulation

  • not expressed if tryptophan is available (MtrB) PubMed
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
  • the RNA switch RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • MtrB: transcription termination, PubMed, in mtrB regulon

• Genes: trpE-trpD-trpC-trpF-trpB-trpA-hisC-tyrA-aroE

  Description: PubMed

  Regulation

  • not expressed if tryptophan is available (MtrB) PubMed
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

  Regulatory mechanism

  • MtrB: transcription termination, PubMed, in mtrB regulon

Biological materials

Mutant

• BKE22680 (trpE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGCTCTCACTCCTTATG,  downstream forward: _UP4_GCTGATGAACAGATTTCTAC
• BKK22680 (trpE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGCTCTCACTCCTTATG,  downstream forward: _UP4_GCTGATGAACAGATTTCTAC

References

Reviews

Loading

Other original publications

Loading

The trpE RNA switch

Loading