psdS

168

two-component sensor kinase, control of psdA-psdB in response to lipid II-binding lantibiotics, such as nisin and gallidermin

Locus: BSU_34710

Molecular weight: 40.02 kDa

Isoelectric point: 7.36

Protein length: 356 aa

Gene length: 1071 bp

Function: resistance against toxic peptides

Product: two-component sensor kinase

Essential: no

Synonyms: psdS, yvcQ

Genomic Context

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List of homologs in different organisms, belongs to COG0642 (Galperin et al., 2021)

Gene

Coordinates: 3,566,358  3,567,428

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of PsdR
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

Domains

• two transmembrane segments
• Histidine kinase domain (aa 133-349) (according to UniProt)

Structure

• 4I5S (PDB) (from Streptococcus mutans, the C-terminal histidine phosphotransferase domain, 29% identity) PubMed
• O06979 (AlphaFold)

Modification

• autophosphorylation on a His residue

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: psdR-psdS-psdA-psdB

  Description: PubMed

  Regulation

  • ''psdA'': induced by nisin, gallidermin (PsdR) PubMed

  Regulatory mechanism

  • PsdR: activation, PubMed, in psdR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B636 (yvcQ::erm), available at the NBRP B. subtilis, Japan
• BKE34710 (psdS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAAGCGGTCGATGAGATAAG,  downstream forward: _UP4_TAAGATTGGGGAGCGGAATT
• BKK34710 (psdS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAAGCGGTCGATGAGATAAG,  downstream forward: _UP4_TAAGATTGGGGAGCGGAATT

References

Reviews

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Original Publications

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