gerE

168

transcriptional regulator (repressor or activator) of a subset of [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]-dependent late spore coat genes

locus: BSU_28410

Molecular weight: 8.43 kDa

pI: 9.64

Protein length: 74 aa

Gene length: 225 bp

function: regulation of [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]-dependent gene expression

product: transcriptional regulator (LuxR-FixJ family)

essential: no

synonyms: gerE

List of homologs in different organisms, belongs to COG5905 (Galperin et al., 2021)

Gene

Coordinates: 2,904,727  2,904,951

Phenotypes of a mutant

• unable to germinate efficiently [Pubmed|3139490]

The protein

Protein family

• LuxR-FixJ family of transcription regulators

[wiki|Domains]

• [wiki|HTH luxR-type domain] (aa 5-70) (according to UniProt)

Structure

• [PDB|1FSE]
• [AF|P11470]

Expression and Regulation

Operons

• genes: [gene|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]

  description: [Pubmed|3114423]

  regulation

  • expressed late during sporulation in the mother cell ([protein|search|SigK], [wiki|SpoIIID]) [Pubmed|15699190,7896717]

  regulatory mechanism

  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: activation, [Pubmed|7896717], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]
  • [protein|E15E9F88B0E03FE8834C646369D2F4B4E713EF41|citB]: mRNA stability control, results in mRNA accumulation [Pubmed|16923907], in [regulon|protein:E15E9F88B0E03FE8834C646369D2F4B4E713EF41|citB regulon]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|15699190], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]

Biological materials

Mutant

• BKE28410 ([gene|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28410 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGTATTGTAACCCTCCTT,  downstream forward: _UP4_TAATCCTTGCCGGTATTCCT
• BKK28410 ([gene|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28410 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGTATTGTAACCCTCCTT,  downstream forward: _UP4_TAATCCTTGCCGGTATTCCT

labs

• [wiki|Arthur Aronson], Purdue University, West Lafayette, USA [http://wwwdev.gradschool.purdue.edu/PULSe/faculty.cfm?fid=5&range=0 homepage]
• [wiki|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]

References

Reviews

• 31350897,35638784

Original Publications

• 7519271,2474075,11418558,10400595,7592393,11741866,7592342,3135411,9852018,1691789,15383836,11150673,1518043,7814326,14523132,12031481,11418558,3114423,7896717,11243786,17890309,15470121,20435725,21670523,23064347,3139490,25259857,6787012,26279233,16923907