psdB

168

[wiki|ABC transporter] (permease) for the export of lipid II-binding lantibiotics, such as nisin and gallidermin

locus: BSU_34690

Molecular weight: 73.22 kDa

pI: 9.8

Protein length: 646 aa

Gene length: 1941 bp

function: export of toxic peptides

product: [wiki|ABC transporter] (permease)

essential: no

synonyms: psdB, yvcS

List of homologs in different organisms, belongs to COG0577 (Galperin et al., 2021)

Gene

Coordinates: 3,563,581  3,565,521

The protein

Protein family

• [wiki|ABC-4 integral membrane protein family] (according to UniProt)

Structure

• [AF|O06981]

Paralogous protein(s)

• [protein|4423383A3F6DAFDFA210F4BEEF7D8CBEC674DCEA|yxdM]

[wiki|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|985E7F25C809AA5DF372101E64960DF704EED53F|psdR]-[gene|A9525886E911164BDFF0A55DA9F5FDD1C1B7A3B6|psdS]-[gene|464DD1855E6B3195EE242637612A16C73FF32FC0|psdA]-[gene|AC7F08DC1DF7DD2F9AFF84041D357B286B1244AC|psdB]

  description: [Pubmed|21078927]

  regulation

  • ''[protein|search|psdA]'': induced by nisin, gallidermin ([protein|search|PsdR]) [Pubmed|21078927]

  regulatory mechanism

  • [protein|985E7F25C809AA5DF372101E64960DF704EED53F|psdR]: activation, [Pubmed|21078927], in [regulon|protein:985E7F25C809AA5DF372101E64960DF704EED53F|psdR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|21078927], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

• genes: [gene|464DD1855E6B3195EE242637612A16C73FF32FC0|psdA]-[gene|AC7F08DC1DF7DD2F9AFF84041D357B286B1244AC|psdB]

  description: [Pubmed|18394148]

  regulation

  • ''[protein|search|psdA]'': induced by nisin, gallidermin ([protein|search|PsdR]) [Pubmed|21078927]

Biological materials

Mutant

• MGNA-B634 (yvcS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1633 NBRP B. subtilis, Japan]
• BKE34690 ([gene|AC7F08DC1DF7DD2F9AFF84041D357B286B1244AC|psdB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34690 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CCGTTGCAGATTCCCAAGAA,  downstream forward: _UP4_TAATATGAAATCCGGTCTGT
• BKK34690 ([gene|AC7F08DC1DF7DD2F9AFF84041D357B286B1244AC|psdB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34690 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CCGTTGCAGATTCCCAAGAA,  downstream forward: _UP4_TAATATGAAATCCGGTCTGT

References

Reviews

• 38869304

Original Publications

• 18394148,10092453,21283517,39397791