ylmD

168

peptidoglycan-editing factor, prevents incorporation of glycine or L-serine into the PG sacculi

Locus: BSU_15370

Molecular weight: 30.79 kDa

Isoelectric point: 6.18

Protein length: 278 aa

Gene length: 837 bp

Function: quality control to maintain composition and integrity of peptidoglycan

Product: peptidoglycan-editing factor

Essential: no

Synonyms: ylmD, pgeF

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1496 (Galperin et al., 2021)

Gene

Coordinates: 1,609,327 → 1,610,163

Phenotypes of a mutant

• sensitive to ß-lactam antibiotics PubMed

The protein

Protein family

• multicopper oxidase YfiH/RL5 family (single member, according to UniProt)

Structure

• 1T8H (PDB) (from Geobacillus stearothermophilus, 52% identity)
• O31726 (AlphaFold)

Expression and Regulation

Operons

• Genes: ylmD-ylmE-sepF-ylmG-ylmH

  Description: PubMed

  Regulation

  • repressed under conditions that trigger sporulation (Spo0A) PubMed

  Regulatory mechanism

  • Spo0A: repression, PubMed, in spo0A regulon

Biological materials

Mutant

• MGNA-B125 (ylmD::erm), available at the NBRP B. subtilis, Japan
• BKE15370 (ΔylmD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATGATCCAACCTTTCG,  downstream forward: _UP4_GGAATGAAGGAGGCATAAAA
• BKK15370 (ΔylmD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATGATCCAACCTTTCG,  downstream forward: _UP4_GGAATGAAGGAGGCATAAAA

References

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