ycnI

168

putative copper chaperone

Locus: BSU_03940

Molecular weight: 21.97 kDa

Isoelectric point: 4.83

Protein length: 204 aa

Gene length: 615 bp

Function: copper homeostasis

Product: copper-binding protein

Essential: no

Synonyms: ycnI

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG4549 (Galperin et al., 2021)

Gene

Coordinates: 446,174  446,788

The protein

Catalyzed reaction/ biological activity

• binds a Cu²⁺ ion PubMed

Domains

• DUF1775 domain PubMed

Structure

• 7ME6 (PDB) apo form PubMed
• 7MEK (PDB) Cu bound form PubMed
• 3ESM (PDB) (from Nocardia farcinica, corresponds to aa 25 ... 137, 34.5% identity)
• P94431 (AlphaFold)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: ycnK-ycnJ-ycnI

  Description: PubMed

  Regulation

  • induced by copper limitation (YcnK) PubMed

  Regulatory mechanism

  • YcnK: repression, PubMed, in ycnK regulon
  • AbrB: repression, PubMed, in abrB regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE03940 (ycnI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACGTAAACACTCCTTAAT,  downstream forward: _UP4_TAATAAAGAAAGCGATCCAG
• BKK03940 (ycnI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACGTAAACACTCCTTAAT,  downstream forward: _UP4_TAATAAAGAAAGCGATCCAG

References

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