xhlB

168

putative holin, required for the formation of membrane vesicles in a sub-population of cells

Locus: BSU_12800

Molecular weight: 9.59 kDa

Isoelectric point: 9.5

Protein length: 87 aa

Gene length: 264 bp

Function: host cell lysis

Product: putative holin

Essential: no

Synonyms: xhlB, xpaB

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 1,347,013  1,347,276

The protein

Protein family

• SPP1 holin family (with BhlB, according to UniProt)

Structure

• Q99163 (AlphaFold)

Localization

• membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: xhlA-xhlB-xlyA

  Description: PubMed

• Genes: xtmA-xtmB-xkdE-xkdF-xkdG-xkdH-xkdI-xkdJ-xkdK-xkdM-xkdN-xkzB-xkdO-xkdP-xkdQ-xkdR-xkdS-xkdT-xkdU-xkzA-xkdV-xkdW-xkdX-xepA-xhlA-xhlB-xlyA

  Description: PubMed

  Regulation

  • expression during spore germination is strongly reduced under conditions of osmotic stress PubMed

  Sigma factors

  • Xpf: sigma factor, PubMed, in xpf regulon

Biological materials

Mutant

• BKE12800 (xhlB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAACGTGTTCATAAAAATCC,  downstream forward: _UP4_CTGACGAAATAAGGAGAGAT
• BKK12800 (xhlB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAACGTGTTCATAAAAATCC,  downstream forward: _UP4_CTGACGAAATAAGGAGAGAT

References

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