pta

168

phosphotransacetylase

Locus: BSU_37660

Molecular weight: 34.64 kDa

Isoelectric point: 4.65

Protein length: 323 aa

Gene length: 972 bp

Function: overflow metabolism

Product: phosphotransacetylase

Essential: no

E.C.: 2.3.1.8

Synonyms: pta, ipa-88d, ywfJ

Genomic Context

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List of homologs in different organisms, belongs to COG0280 (Galperin et al., 2021)

Gene

Coordinates: 3,865,355  3,866,326

Phenotypes of a mutant

• the mutant tends to acquire suppressor mutations that result in improved growth PubMed

The protein

Catalyzed reaction/ biological activity

• acetyl-CoA + phosphate --> acetyl phosphate + CoA (according to UniProt)

Protein family

• phosphate acetyltransferase and butyryltransferase family (with Ptb, according to UniProt)

Structure

• 1XCO (PDB) (complex with acetylphosphate)PubMed
• 1TD9 (PDB)
• P39646 (AlphaFold)

Modification

• phosphorylation on (Ser-123 OR Thr-128 OR Ser-129) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: pta

  Description: PubMed

  Regulation

  • expression activated by glucose (2.6 fold) (CcpA) PubMed

  Regulatory mechanism

  • CcpA: activation, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• GP668 (aphA3), available in Jörg Stülke's lab
• BKE37660 (pta::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAATAAAACCTCCTCAAA,  downstream forward: _UP4_TAATAAAATTGAAGACAATG
• BKK37660 (pta::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAATAAAACCTCCTCAAA,  downstream forward: _UP4_TAATAAAATTGAAGACAATG

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

References

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