pucL

168

uricase, urate oxidase

Locus: BSU_32450

Molecular weight: 56.39 kDa

Isoelectric point: 5.48

Protein length: 494 aa

Gene length: 1485 bp

Function: purine utilization

Product: uricase

Essential: no

E.C.: 1.7.3.3

Synonyms: pucL, yunL

Genomic Context

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List of homologs in different organisms, belongs to COG3648 (Galperin et al., 2021)

Gene

Coordinates: 3,333,162  3,334,646

The protein

Catalyzed reaction/ biological activity

• 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H⁺ --> (S)-allantoin + CO₂ (according to UniProt)
• H₂O + O₂ + urate --> 5-hydroxyisourate + H₂O₂ (according to UniProt)

Protein family

• N-terminal part: OHCU decarboxylase family (single member, according to UniProt)
• C-terminal part: uricase family (single member, according to UniProt)

Structure

• 6A4M (PDB)
• O32141 (AlphaFold)

Expression and Regulation

Operons

• Genes: pucJ-pucK-pucL-pucM

  Description: PubMed

  Regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

  Regulatory mechanism

  • TnrA: activation, PubMed, in tnrA regulon
  • PucR: activation, PubMed, in pucR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: pucR-pucJ-pucK-pucL-pucM

  Description: PubMed

  Regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

  Regulatory mechanism

  • PucR: auto-repression, PubMed, in pucR regulon
  • TnrA: activation, PubMed, in tnrA regulon

Biological materials

Mutant

• MGNA-A937 (yunL::erm), available at the NBRP B. subtilis, Japan
• BKE32450 (pucL::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTGAACATTGGCATCT,  downstream forward: _UP4_AAATGTCGGAGCCTGAAAGC
• BKK32450 (pucL::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTGAACATTGGCATCT,  downstream forward: _UP4_AAATGTCGGAGCCTGAAAGC

References

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