gyrB

168

DNA gyrase (subunit B)

Locus: BSU_00060

Molecular weight: 71.32 kDa

Isoelectric point: 5.38

Protein length: 638 aa

Gene length: 1917 bp

Function: DNA supercoiling, control of replication initiation, resolution of head-on DNA replication-Transcription conflicts

Product: DNA gyrase (subunit B)

Essential: yes

E.C.: 5.99.1.3

Synonyms: gyrB, novA

Genomic Context

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List of homologs in different organisms, belongs to COG0187 (Galperin et al., 2021)

Gene

Coordinates: 4,867  6,783

Phenotypes of a mutant

• essential PubMed
• enhanced colony wrinkling upon knockdown PubMed

The protein

Catalyzed reaction/ biological activity

• ATP-dependent breakage, passage and rejoining of double-stranded DNA (according to UniProt)
• decreases DnaA association with the oriC and inhibits replication initiation PubMed

Protein family

• type II topoisomerase GyrB family (together with ParE) (according to UniProt)

Domains

• TOPRIM domain (aa 422-536) PubMed

Structure

• 4I3H (PDB) (from Streptococcus pneumoniae, 55% identity) PubMed
• 9GBV (PDB) (E. coli DNA gyrase with chirally wrapped 217 bp DNA fragment) PubMed
• 9GGQ (PDB) (E. coli DNA gyrase with chirally wrapped 217 bp DNA fragment in the presence of the fluoroquinolone moxifloxacin) PubMed
• P05652 (AlphaFold)

Modification

• phosphorylated on Ser-400 PubMed

Effectors of protein activity

• novobiocin binds to the ATP binding site of GyrB and inhibits ATP hydrolysis and enzymatic activity, this results in replication overinitiation PubMed
• an R138L mutation confers novobiocin resistance PubMed

Paralogous protein(s)

• ParE

Localization

• nucleoid-associated in actively growing cells PubMed

Expression and Regulation

Operons

• Genes: yaaA-recF-remB-gyrB-gyrA

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Labs working on this gene/protein

• Dagmar Klostermeier, University of Mnster, Germany homepage

References

Reviews

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Original Publications

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