trmR

168

tRNA wobble uridine modifying methyltransferase

Locus: BSU_27360

Molecular weight: 24.92 kDa

Isoelectric point: 5.57

Protein length: 217 aa

Gene length: 654 bp

Function: introduction of 5-methoxyuridine modification in tRNA (U34)

Product: tRNA wobble uridine modifying methyltransferase

Essential: no

Synonyms: trmR, yrrM

Genomic Context

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List of homologs in different organisms, belongs to COG4122 (Galperin et al., 2021)

Gene

Coordinates: 2,795,082 → 2,795,735

The protein

Catalyzed reaction/ biological activity

• methylation of (5-hydroxyuridine) ho⁵U containing tRNA to 5-methoxyuridine (mo⁵U) PubMed
• 5-hydroxyuridine³⁴ in tRNA + S-adenosyl-L-methionine --> 5-methoxyuridine³⁴ in tRNA + H⁺ + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• Methyltransferase superfamily (according to UniProt)
• class I-like SAM-binding methyltransferase superfamily (according to UniProt)

Cofactors

• SAM PubMed

Structure

• 5ZW3 (PDB) PubMed
• 5ZW4 (PDB) (bound to tRNA) PubMed
• O32036 (AlphaFold)

Expression and Regulation

Operons

• Genes: trmR-yrrN-yrrO-udk-greA

  Description: PubMed

Biological materials

Mutant

• MGNA-A853 (yrrM::erm), available at the NBRP B. subtilis, Japan
• BKE27360 (ΔtrmR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTACAAACAGCCTCCCGT,  downstream forward: _UP4_AGTAAAAAGAAGAGGTGAAC
• BKK27360 (ΔtrmR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTACAAACAGCCTCCCGT,  downstream forward: _UP4_AGTAAAAAGAAGAGGTGAAC

References

Research papers

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