yneA

168

inhibits cell division during SOS response

Locus: BSU_17860

Molecular weight: 11.75 kDa

Isoelectric point: 4.61

Protein length: 105 aa

Gene length: 318 bp

Function: inhibits cell division during SOS response

Product: checkpoint enforcement protein

Essential: no

Synonyms: yneA

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 1,918,406  1,918,723

Phenotypes of a mutant

• increased sensitivity to DNA-damaging agents PubMed
• inactivation allows growth of a whiA parB double mutant PubMed

The protein

Protein family

• YneA family (single member, according to UniProt)

Domains

• contains a N-acetylglucosamine-polymer-binding LysM domain (aa 38-89) PubMed

Structure

• Q45056 (AlphaFold)

Localization

• secreted to the medium (signal peptide) PubMed

Additional information

• the activity can be bypassed by specific variants of FtsW PubMed

Expression and Regulation

Operons

• Genes: yneA-yneB-ynzC

  Description: PubMed

  Regulation

  • induced by DNA damage (LexA) PubMed

  Regulatory mechanism

  • LexA: repression, PubMed, in lexA regulon

  Additional information

  • YneA is rapidly degraded by extracellular proteases (CtpA) PubMed

Biological materials

Mutant

• MGNA-B388 (yneA::erm), available at the NBRP B. subtilis, Japan
• BKE17860 (yneA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAACCTCCAACAGGAATG,  downstream forward: _UP4_AGATAGAGAGGAAAGAGAGA
• BKK17860 (yneA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAACCTCCAACAGGAATG,  downstream forward: _UP4_AGATAGAGAGGAAAGAGAGA

References

Reviews

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Original Publications

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