yfkM

168

glyoxalase III-like enzyme, general stress protein, survival of salt, paraquat and ethanol stresses

Locus: BSU_07850

Molecular weight: 18.72 kDa

Isoelectric point: 4.72

Protein length: 172 aa

Gene length: 519 bp

Function: detoxification of methylglyoxal

Product: glyoxalase III-like enzyme

Essential: no

Synonyms: yfkM

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0693 (Galperin et al., 2021)

Gene

Coordinates: 859,745 → 860,263

The protein

Catalyzed reaction/ biological activity

• methylglyoxal -→ D-lactate PubMed

Protein family

• peptidase C56 family (according to UniProt)

Domains

• PfpI endopeptidase domain (aa 3-171) (according to UniProt)

Structure

• 1OI4 (PDB) (from E. coli, 64% identity)
• P80876 (AlphaFold)

Paralogous protein(s)

• YraA

Expression and Regulation

Operons

• Genes: yfkM

  Description: PubMed

  Regulation

  • induced by stress (SigB) PubMed

  Regulatory mechanism

  • Fur: repression, PubMed, in fur regulon

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon

Biological materials

Mutant

• MGNA-C264 (yfkM::erm), available at the NBRP B. subtilis, Japan
• BKE07850 (ΔyfkM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATTCATAACCTCCCGC,  downstream forward: _UP4_TAAGAAAAAACGGACGCTCT
• BKK07850 (ΔyfkM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATTCATAACCTCCCGC,  downstream forward: _UP4_TAAGAAAAAACGGACGCTCT

References

Loading