pheS

168

phenylalanyl-tRNA synthetase (alpha subunit), universally conserved protein

Locus: BSU_28640

Molecular weight: 38.52 kDa

Isoelectric point: 5.08

Protein length: 344 aa

Gene length: 1035 bp

Function: translation

Product: phenylalanyl-tRNA synthetase (alpha subunit)

Essential: yes

E.C.: 6.1.1.20

Synonyms: pheS

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0016 (Galperin et al., 2021)

Gene

Coordinates: 2,929,438 2,930,472

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + L-phenylalanine + tRNA^Phe --> AMP + diphosphate + H⁺ + L-phenylalanyl-tRNA^Phe (according to UniProt)

Protein family

• Class-II aminoacyl-tRNA synthetase family (according to UniProt)

Structure

• 1PYS (PDB) (the PheS-PheT complex from Thermus thermophilus hb8 mutant, 37% identity, 56% similarity) PubMed
• P17921 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• Universally conserved protein
• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: pheS-pheT

  Description: PubMed

  Regulation

  • induced by phenylalanine limitation (T-box) PubMed
  • the T-box RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • T-box: RNA switch, via tRNA controlled RNA switch, in T-box

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

References

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