levE

168

fructose-specific permease of the phosphotransferase system, EIIB of the PTS, Trigger enzyme, control of LevR activity

Locus: BSU_27060

Molecular weight: 17.94 kDa

Isoelectric point: 9.44

Protein length: 162 aa

Gene length: 489 bp

Function: fructose uptake and phosphorylation, control of LevR activity

Product: fructose-specific PTS, EII component

Essential: no

E.C.: 2.7.1.69

Synonyms: levE

Genomic Context

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List of homologs in different organisms, belongs to COG3444 (Galperin et al., 2021)

Gene

Coordinates: 2,761,907 → 2,762,395

The protein

Catalyzed reaction/ biological activity

• D-fructose + N^π-phospho-L-histidyl-[protein] --> D-fructose 1-phosphate + L-histidyl-[protein] (according to UniProt)

Protein family

• PTS permease, mannose family PubMed

Domains

• PTS EIIB domain type-4 (aa 1-163) (according to UniProt)

Structure

• 1BLE (PDB)
• P26380 (AlphaFold)

Localization

• cytoplasm PubMed

Expression and Regulation

Operons

• Genes: levD-levE-levF-levG-sacC

  Description: PubMed

  Regulation

  • induced in the presence of fructose (LevR) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon
  • LevR: activation, PubMed, in levR regulon

  Sigma factors

  • SigL: sigma factor, PubMed, in sigL regulon

Biological materials

Mutant

• BKE27060 (ΔlevE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCATAATTCATCCTCTCT,  downstream forward: _UP4_ACAAAATAATCAAGGGGATG
• BKK27060 (ΔlevE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCATAATTCATCCTCTCT,  downstream forward: _UP4_ACAAAATAATCAAGGGGATG

References

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