guaA

168

GMP synthetase (glutamine-hydrolysing)

Locus: BSU_06360

Molecular weight: 57.78 kDa

Isoelectric point: 4.75

Protein length: 513 aa

Gene length: 1542 bp

Function: biosynthesis of GMP

Product: GMP synthetase (glutamine-hydrolysing)

Essential: no

E.C.: 6.3.5.2

Synonyms: guaA, guaB

Genomic Context

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Categories containing this gene/protein

Cellular processes, Cell envelope and cell division, Cell shape

Metabolism, Nucleotide metabolism, Biosynthesis/ acquisition of nucleotides, Biosynthesis/ acquisition of purine nucleotides

List of homologs in different organisms, belongs to COG0519 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon

Gene

Coordinates: 692,740 694,281

Phenotypes of a mutant

the mutant cells are thinner and shorter than wild type cells PubMed

The protein

Catalyzed reaction/ biological activity

ATP + H₂O + L-glutamine + XMP --> AMP + diphosphate + GMP + 2 H⁺ + L-glutamate (according to UniProt)

Domains

Glutamine amidotransferase type-1 domain (aa 8-198) (according to UniProt)
GMPS ATP-PPase domain (aa 199-388) (according to UniProt)

Structure

2YWB (PDB) (from Thermus thermophilus hb8, 45% identity, 57% similarity)
P29727 (AlphaFold)

Effectors of protein activity

the enzyme is inhibited by guanosine tetraphosphate (stringent response) PubMed

Additional information

belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

Genes: guaA

Description: PubMed

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

MGNA-A918 (yebB::erm), available at the NBRP B. subtilis, Japan
BKE06360 (guaA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGTTGTCACCTAATCTC, downstream forward: _UP4_TAAGAATCAATTAATGGAAA
BKK06360 (guaA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGTTGTCACCTAATCTC, downstream forward: _UP4_TAAGAATCAATTAATGGAAA

Expression vectors

pGP2898: expression of Strep-guaA by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab

References

Reviews

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Original Publications

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