ctc

168

general stress protein, non-essential ribosomal protein BL25

Locus: BSU_00520

Molecular weight: 21.91 kDa

Isoelectric point: 4.22

Protein length: 204 aa

Gene length: 615 bp

Function: translation (under stress conditions)

Product: ribosomal protein BL25

Essential: no

Synonyms: ctc, rplY

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1825 (Galperin et al., 2021)

Gene

Coordinates: 58,783  59,397

The protein

Protein family

• Bacterial ribosomal protein bL25 family (single member, according to UniProt)

Structure

• 4WCE (PDB) (from Staphylococcus aureus, 49.4% identity) PubMed
• 1FEU (PDB) (from Thermus thermophilus, 32% identity, 53% similarity) PubMed
• P14194 (AlphaFold)

Localization

• in the ribosome (large subunit) PubMed

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and Regulation

Operons

• Genes: gcaD-prs-ctc

  Description: PubMed

  Regulatory mechanism

  • stringent response: negative regulation, PubMed, in stringent response

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: ctc-pth-fin

  Description: PubMed

  Regulation

  • induced under stress conditions (SigB) PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon

• Genes: ctc

  Description: PubMed

  Regulation

  • induced under stress conditions (SigB) PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon

Additional information

• translation is substantially enhanced when the gene is transcribed by SigB-RNA polymerase PubMed

Biological materials

Mutant

• MGNA-B912 (ctc::erm), available at the NBRP B. subtilis, Japan
• GP500(spc), available in Jörg Stülke's lab PubMed
• CS206 (''ctc::aphA3'', available in Colin Harwood's and Jörg Stülke's labs) PubMed
• BKE00520 (ctc::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTCAGCACCATCCTCT,  downstream forward: _UP4_TAATAGCTTAAGGCGTAACC
• BKK00520 (ctc::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTCAGCACCATCCTCT,  downstream forward: _UP4_TAATAGCTTAAGGCGTAACC

Expression vectors

• expression/ purification with N-terminal His-tag in E. coli: pGP804 (in pWH844), available in Jörg Stülke's lab PubMed

Antibody

• available in Jörg Stülke's lab PubMed

References

Reviews

Loading

Original Publications

Loading