araL

168

sugar phosphate phosphatase, L-arabinose operon

Locus: BSU_28770

Molecular weight: 29.16 kDa

Isoelectric point: 5.37

Protein length: 272 aa

Gene length: 819 bp

Function: detoxification of accidental accumulation of phosphorylated metabolites

Product: sugar phosphate phosphatase

Essential: no

Synonyms: araL, yseA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0647 (Galperin et al., 2021)

Gene

Coordinates: 2,944,187 → 2,945,005

The protein

Catalyzed reaction/ biological activity

• H₂O + O-phospho-serine --> L-serine + phosphate (according to UniProt)

Protein family

• HAD superfamily (according to UniProt)

Structure

• 3QGM (PDB) (from Archaeoglobus Fulgidus (35%) )
• P94526 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: araA-araB-araD-araL-araM-araN-araP-araQ-abfA

  Description: PubMed

  Regulation

  • induced by arabinose (AraR) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon
  • AraR: repression, PubMed, in araR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

Mutant

• MGNA-B015 (araL::erm), available at the NBRP B. subtilis, Japan
• BKE28770 (ΔaraL::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATGCCAGCCGGTGACACAG,  downstream forward: _UP4_TTGGCTGAGGAGGGGATTCT
• BKK28770 (ΔaraL::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATGCCAGCCGGTGACACAG,  downstream forward: _UP4_TTGGCTGAGGAGGGGATTCT

References

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