iolJ

168

2-deoxy-5-keto-gluconic acid-6-phosphate aldolase, formation of dihydroxyacetone phosphate and malonic semialdehyde (6th reaction)

Locus: BSU_39670

Molecular weight: 31.22 kDa

Isoelectric point: 4.85

Protein length: 290 aa

Gene length: 873 bp

Function: myo-inositol catabolism

Product: 2-deoxy-5-keto-gluconic acid-6-phosphate aldolase, formation of dihydroxyacetone phosphate and malonic semialdehyde (6th reaction)

Essential: no

E.C.: 4.1.2.29

Synonyms: iolJ, fbaB, yxdI

Genomic Context

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Categories containing this gene/protein

Metabolism, Carbon metabolism, Utilization of specific carbon sources, Utilization of inositol

List of homologs in different organisms, belongs to COG0191 (Galperin et al., 2021)

Gene

Coordinates: 4,073,081  4,073,953

The protein

Catalyzed reaction/ biological activity

6-phospho-5-dehydro-2-deoxy-D-gluconate --> 3-oxopropanoate + dihydroxyacetone phosphate (according to UniProt)

Protein family

class II fructose-bisphosphate aldolase family (with FbaA, according to UniProt)

Structure

4TO8 (PDB) (from Staphylococcus aureus, 56% identity) PubMed
P42420 (AlphaFold)

Effectors of protein activity

inhibited by alpha-keto acids PubMed

Paralogous protein(s)

FbaA

Expression and Regulation

Operons

Genes: iolA-iolB-iolC-iolD-iolE-iolF-iolG-iolH-iolI-iolJ

Description: PubMed

Regulation

induced by inositol (IolR) PubMed

Regulatory mechanism

IolR: repression, PubMed, in iolR regulon
CcpA: repression, PubMed, in ccpA regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

MGNA-B701 (iolJ::erm), available at the NBRP B. subtilis, Japan
BKE39670 (iolJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTCACCCCTCCTTTAT,  downstream forward: _UP4_TAAAGAGACCAGAAACCGCC
BKK39670 (iolJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTCACCCCTCCTTTAT,  downstream forward: _UP4_TAAAGAGACCAGAAACCGCC

Labs working on this gene/protein

Yasutaro Fujita, University of Fukuyama, Japan
[[Ken-ichi Yoshida]], Kobe University, Japan

References

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