bsdB

168

phenolic acid decarboxylase

locus: BSU_03630

Molecular weight: 22.39 kDa

pI: 10.06

Protein length: 204 aa

Gene length: 615 bp

function: resistance to salicylic acid

product: phenolic acid decarboxylase

essential: no

synonyms: bsdB, yclB

List of homologs in different organisms, belongs to COG0163 (Galperin et al., 2021)

Gene

Coordinates: 412,540  413,154

The protein

Catalyzed reaction/ biological activity

• conversion of vanillic acid to guaiacol [Pubmed|26658822]
• dimethylallyl phosphate + FMNH₂ --> phosphate + prenyl-FMNH₂ (according to UniProt)

Protein family

• UbiX/PAD1 family (single member, according to UniProt)

Structure

• [PDB|1SBZ] (from E. coli, 57% identity) [pubmed|15459342]
• [AF|P94404]

Expression and Regulation

Operons

• genes: [gene|8A83E44AFE9A1A635C65E6B526690AF7DA201BE1|bsdB]-[gene|FD23F4C2AFCBFE35A79116C5A707FACD454E4180|bsdC]-[gene|868DC9DE1C9C26C780F4CEAA4185640B5B6B781B|bsdD]

  description: [Pubmed|17295427]

  regulation

  • induced by salicylic acid ([protein|search|BsdA]) [Pubmed|17295427]

  regulatory mechanism

  • [protein|28D03575FA24525C162E6C161631034568E89622|bsdA]: activation, [Pubmed|17295427], in [regulon|protein:28D03575FA24525C162E6C161631034568E89622|bsdA regulon]

Biological materials

Mutant

• MGNA-C061 (yclB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2059 NBRP B. subtilis, Japan]
• BKE03630 ([gene|8A83E44AFE9A1A635C65E6B526690AF7DA201BE1|bsdB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03630 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCAATCATACTCCTGA,  downstream forward: _UP4_AAACAAAAAGGAGGAGCTTG
• BKK03630 ([gene|8A83E44AFE9A1A635C65E6B526690AF7DA201BE1|bsdB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03630 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCAATCATACTCCTGA,  downstream forward: _UP4_AAACAAAAAGGAGGAGCTTG

References

• 15979273,17295427,26658822,15459342