ligA

168

DNA ligase (NAD-dependent)

Locus: BSU_06620

Molecular weight: 74.69 kDa

Isoelectric point: 4.71

Protein length: 668 aa

Gene length: 2007 bp

Function: replication

Product: DNA ligase (NAD-dependent)

Essential: yes

E.C.: 6.5.1.2

Synonyms: ligA, lig, yerG

Genomic Context

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List of homologs in different organisms, belongs to COG0272 (Galperin et al., 2021)

Gene

Coordinates: 721,613  723,619

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• NAD(+) + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) --> (deoxyribonucleotide)(n+m) + AMP + beta-nicotinamide D-nucleotid (according to UniProt)

Protein family

• NAD-dependent DNA ligase family (single member, according to UniProt)

Domains

• BRCT domain (aa 590-668) (according to UniProt)

Structure

• 1B04 (PDB) (adenylation domain, Geobacillus stearothermophilus)
• O31498 (AlphaFold)

Expression and Regulation

Operons

• Genes: pcrB-pcrA-ligA-yerH

  Description: PubMed

  Regulation

  • induced by DNA damage (LexA) PubMed

  Regulatory mechanism

  • LexA: repression, PubMed, in lexA regulon

Biological materials

Mutant

• MGNA-B444 (yerG::erm), available at the NBRP B. subtilis, Japan

References

Reviews

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Original Publications

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