glpF

168

glycerol facilitator

Locus: BSU_09280

Molecular weight: 28.59 kDa

Isoelectric point: 9.51

Protein length: 274 aa

Gene length: 825 bp

Function: glycerol uptake

Product: glycerol facilitator

Essential: no

Synonyms: glpF

Genomic Context

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List of homologs in different organisms, belongs to COG0580 (Galperin et al., 2021)

Gene

Coordinates: 1,002,501 → 1,003,325

The protein

Catalyzed reaction/ biological activity

• uptake of glycerol

Protein family

• MIP/aquaporin (TC 1.A.8) family (single member, according to UniProt)

Structure

• 1FX8 (PDB) (the protein from E. coli, 37% identity) PubMed
• P18156 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: glpF-glpK

  Description: PubMed

  Regulation

  • induction by glycerol (GlpP) PubMed
  • repressed under anaerobic conditions (ResD) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon
  • GlpP: antitermination, /antitermination via GlpP-dependent RNA switch, in glpP regulon
  • AbrB: activation, PubMed, in abrB regulon
  • ResD: repression, PubMed, in resD regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Additional information

• increased expression at increased temperature (via an RNA thermometer that covers the Ribosomal binding site of the mRNA) PubMed

Biological materials

Mutant

• GP99 (cat) (available in Jörg Stülke's lab)
• BKE09280 (ΔglpF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGCACATTCCTCCTAAA,  downstream forward: _UP4_ATTTAATCAAAGGGGAGACA
• BKK09280 (ΔglpF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGCACATTCCTCCTAAA,  downstream forward: _UP4_ATTTAATCAAAGGGGAGACA

References

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