glnT

168

glutamine transporter (proton symport)

Locus: BSU_02420

Molecular weight: 49.83 kDa

Isoelectric point: 10.13

Protein length: 478 aa

Gene length: 1437 bp

Function: glutamine uptake

Product: glutamine transporter (proton symport)

Essential: no

Synonyms: glnT, ybgH

Genomic Context

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List of homologs in different organisms, belongs to COG1115 (Galperin et al., 2021)

Gene

Coordinates: 262,732 → 264,168

The protein

Protein family

• Alanine or glycine:cation symporter (AGCS) (TC 2.A.25) family (according to UniProt)

Structure

• 6CSE (PDB) (from Methanococcus maripaludis), 37.4% identity PubMed
• O31464 (AlphaFold)

Paralogous protein(s)

• AlsT, YflA, YrbD

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: glsA-glnT

  Description: PubMed

  Regulation

  • induced in the presence of glutamine (GlnL) PubMed

  Regulatory mechanism

  • GlnL: activation, PubMed, in glnL regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• GP3503 (ΔglnT::kan), available in Jörg Stülke's lab
• MGNA-B942 (glnT::erm), available at the NBRP B. subtilis, Japan
• BKE02420 (ΔglnT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGAATCACCTCTTTTTT,  downstream forward: _UP4_TAAGAAAAGGGTGTTCAGAC
• BKK02420 (ΔglnT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGAATCACCTCTTTTTT,  downstream forward: _UP4_TAAGAAAAGGGTGTTCAGAC

References

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