rsbQ

168

butenolide hydrolase,alpha/beta hydrolase, required for RsbP activation

Locus: BSU_34100

Molecular weight: 29.87 kDa

Isoelectric point: 5

Protein length: 269 aa

Gene length: 810 bp

Function: control of SigB activity in response to plant metabolites

Product: butenolide hydrolase

Essential: no

Synonyms: rsbQ, yvfQ

Genomic Context

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List of homologs in different organisms, belongs to COG0596 (Galperin et al., 2021)

Gene

Coordinates: 3,499,541  3,500,350

Phenotypes of a mutant

• no induction of σ^B-dependent transcripts in response to carbon limitation

The protein

Protein family

• AB hydrolase superfamily (according to UniProt)

Domains

• AB hydrolase-1 domain (aa 23-259) (according to InterPro)

Structure

• 1WOM (PDB) PubMed
• O07015 (AlphaFold)

Expression and Regulation

Operons

• Genes: rsbP-rsbQ

  Description: PubMed

Biological materials

Mutant

• MGNA-A480 (yvfQ::erm), available at the NBRP B. subtilis, Japan
• BKE34100 (rsbQ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATTGGCTCCTTTCAGC,  downstream forward: _UP4_TGATATAGGAACGAAAGGGA
• BKK34100 (rsbQ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATTGGCTCCTTTCAGC,  downstream forward: _UP4_TGATATAGGAACGAAAGGGA

Labs working on this gene/protein

• Chet Price, Davis, USA homepage

References

Reviews

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Original Publications

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