hutM

168

histidine permease

Locus: BSU_39390

Molecular weight: 51.41 kDa

Isoelectric point: 9.01

Protein length: 475 aa

Gene length: 1428 bp

Function: histidine uptake

Product: histidine permease

Essential: no

Synonyms: hutM

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0833 (Galperin et al., 2021)

Gene

Coordinates: 4,047,538  4,048,965

The protein

Protein family

• amino acid-polyamine-organocation (APC) superfamily (according to UniProt)

Structure

• 7CMH (PDB) (human protein, corresponds to aa 23 ... 356, 25% identity) PubMed
• P42087 (AlphaFold)

Paralogous protein(s)

• YvbW, AimB, RocC, RocE, YbgF, YdgF, AapA, GabP, AlaP

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: hutP-hutH-hutU-hutI-hutG-hutM

  Description: PubMed

  Regulation

  • induced by histidine (HutP) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon
  • CodY: repression, PubMed, in codY regulon
  • HutP: antitermination, at a protein-dependent RNA switch located between hutP and hutH PubMed, in hutP regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• GP3609 (phleo), available in Jörg Stülke's lab
• MGNA-B783 (hutM::erm), available at the NBRP B. subtilis, Japan
• BKE39390 (hutM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTGTCTCGCCCTCCTTTT,  downstream forward: _UP4_TAATTAAAAAAGCACACCCC
• BKK39390 (hutM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTGTCTCGCCCTCCTTTT,  downstream forward: _UP4_TAATTAAAAAAGCACACCCC

References

Reviews

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Original Publications

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