ytrB

168

[wiki|ABC transporter] (ATP-binding protein)

locus: BSU_30450

Molecular weight: 33.67 kDa

pI: 5.51

Protein length: 292 aa

Gene length: 879 bp

function: unknown

product: [wiki|ABC transporter] (ATP-binding protein)

essential: no

synonyms: ytrB

List of homologs in different organisms, belongs to COG1131 (Galperin et al., 2021)

Gene

Coordinates: 3,117,976 → 3,118,854

The protein

Protein family

• [wiki|ABC transporter superfamily] (according to UniProt)

[wiki|Domains]

• [wiki|ABC transporter domain] (aa 2-227) (according to UniProt)

Structure

• [PDB|1VPL] (from Thermotoga maritima, 31% identity)
• [AF|O34641]

[wiki|Localization]

• cell membrane (via [protein|31977FDC549E45911C333623DCA3D8941A93D8FF|ytrC]-[protein|9C8233C48733A1E9271EE7AA1283EA9F446CF9DA|ytrD]) [Pubmed|10986249]

Expression and Regulation

Operons

• genes: [gene|DCFA6025F7B4D7C5F6FB8107DDA6538CED33084D|ytrG]-[gene|6A074CE9AD98A2751A216A2EA8156ACE6FFC7280|ytrA]-[gene|BF81AD95FE6CF7662D012EF293C769E80C093D34|ytrB]-[gene|31977FDC549E45911C333623DCA3D8941A93D8FF|ytrC]-[gene|9C8233C48733A1E9271EE7AA1283EA9F446CF9DA|ytrD]-[gene|4FE6A51A0D9BBBF574EA2EF18B95B5BFC0A64CCB|ytrE]-[gene|9024AB63030C1934A954EE6A378CADBDC977E863|ytrF]

  description: [Pubmed|10986249,21856850]

  regulation

  • expressed early in the stationary phase [Pubmed|10986249]

  regulatory mechanism

  • [protein|6A074CE9AD98A2751A216A2EA8156ACE6FFC7280|ytrA]: repression, [Pubmed|10986249,21856850], in [regulon|protein:6A074CE9AD98A2751A216A2EA8156ACE6FFC7280|ytrA regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|10986249,21856850], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• GP3188 (Δ[gene|BF81AD95FE6CF7662D012EF293C769E80C093D34|ytrB]), available in [wiki|Jörg Stülke]'s lab [pubmed|33897624]
• GP2646 Δ([gene|DCFA6025F7B4D7C5F6FB8107DDA6538CED33084D|ytrG]-[gene|6A074CE9AD98A2751A216A2EA8156ACE6FFC7280|ytrA]-[gene|BF81AD95FE6CF7662D012EF293C769E80C093D34|ytrB]-[gene|31977FDC549E45911C333623DCA3D8941A93D8FF|ytrC]-[gene|9C8233C48733A1E9271EE7AA1283EA9F446CF9DA|ytrD]-[gene|4FE6A51A0D9BBBF574EA2EF18B95B5BFC0A64CCB|ytrE]-[gene|9024AB63030C1934A954EE6A378CADBDC977E863|ytrF])::''ermC'', available in [wiki|Jörg Stülke]'s lab [pubmed|33897624]
• MGNA-A131 (ytrB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/131 NBRP B. subtilis, Japan]
• BKE30450 (Δ[gene|BF81AD95FE6CF7662D012EF293C769E80C093D34|ytrB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30450 BGSC] and in [wiki|Jörg Stülke]'s lab,  [Pubmed|28189581], upstream reverse: _UP1_GTTCAATCATTTTTTTTGCC,  downstream forward: _UP4_AGATGGTAGACCGAGGTCTC
• BKK30450 (Δ[gene|BF81AD95FE6CF7662D012EF293C769E80C093D34|ytrB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTTCAATCATTTTTTTTGCC,  downstream forward: _UP4_AGATGGTAGACCGAGGTCTC

References

• 36815589,10986249,10092453,12399512,33450852,33897624