ytrB

168

ABC transporter (ATP-binding protein)

Locus: BSU_30450

Molecular weight: 33.67 kDa

Isoelectric point: 5.51

Protein length: 292 aa

Gene length: 879 bp

Function: unknown

Product: ABC transporter (ATP-binding protein)

Essential: no

Synonyms: ytrB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1131 (Galperin et al., 2021)

Gene

Coordinates: 3,117,976 → 3,118,854

The protein

Protein family

• ABC transporter superfamily (according to UniProt)

Domains

• ABC transporter domain (aa 2-227) (according to UniProt)

Structure

• 1VPL (PDB) (from Thermotoga maritima, 31% identity)
• O34641 (AlphaFold)

Localization

• cell membrane (via YtrC-YtrD) PubMed

Expression and Regulation

Operons

• Genes: ytrG-ytrA-ytrB-ytrC-ytrD-ytrE-ytrF

  Description: PubMed

  Regulation

  • expressed early in the stationary phase PubMed

  Regulatory mechanism

  • YtrA: repression, PubMed, in ytrA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• GP3188 (ΔytrB), available in Jörg Stülke's lab PubMed
• GP2646 Δ(ytrG-ytrA-ytrB-ytrC-ytrD-ytrE-ytrF)::ermC, available in Jörg Stülke's lab PubMed
• MGNA-A131 (ytrB::erm), available at the NBRP B. subtilis, Japan
• BKE30450 (ΔytrB::erm  trpC2) available at BGSC and in Jörg Stülke's lab,  PubMed, upstream reverse: _UP1_GTTCAATCATTTTTTTTGCC,  downstream forward: _UP4_AGATGGTAGACCGAGGTCTC
• BKK30450 (ΔytrB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTTCAATCATTTTTTTTGCC,  downstream forward: _UP4_AGATGGTAGACCGAGGTCTC

References

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