sndA

168

[metabolite|N-acetylcysteine] deacetylase, required for the conversion of S-methyl-cysteine to cysteine

locus: BSU_29290

Molecular weight: 45.08 kDa

pI: 5.55

Protein length: 416 aa

Gene length: 1251 bp

function: utilization of S-methyl-cysteine

product: [metabolite|N-acetylcysteine] deacetylase

essential: no

synonyms: sndA, hipO, ytnL

List of homologs in different organisms, belongs to COG1473 (Galperin et al., 2021)

Gene

Coordinates: 2,999,717  3,000,967

Phenotypes of a mutant

• a [gene|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA] [gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB] [gene|D1A8D231369F0BF48639B61094B3222EC4605B79|sndC] triple mutant does not grow with [metabolite|N-acetylcysteine] and [metabolite|S-(2-succino)-L-cysteine] as the single sources of sulfur [Pubmed|23944997,29626092]

The protein

Catalyzed reaction/ biological activity

• [metabolite|N-acetylcysteine] + [metabolite|oxaloacetate] --> [metabolite|cysteine] + [metabolite|acetate] [Pubmed|41260340,35584544]

Protein family

• [wiki|peptidase M20 family] (according to UniProt)

[wiki|Cofactors]

• Co²⁺ [Pubmed|41260340], Mn²⁺ [Pubmed|41260340]

Structure

• [PDB|1YSJ] ([protein|C99AA3FDBB561BCED60915078DC746690C071753|sndB], 48% identity)
• [AF|O34980]

Kinetic information

• K_M for [metabolite|N-acetylcysteine]: 855.0 ± 339.2 µM [Pubmed|41260340]
• V_Max: 43.0 ± 6.7 µmol min^-1 mg^-1 [Pubmed|41260340]

Paralogous protein(s)

• [protein|C99AA3FDBB561BCED60915078DC746690C071753|sndB], [protein|D1A8D231369F0BF48639B61094B3222EC4605B79|sndC], [protein|D6F1906CE8386F78F451D6B794F74DE4AD25AC9C|ykuR]

Expression and Regulation

Operons

• genes: [gene|E3F1B3A4086DBC40FD9AE9CD6981270FBDFA6CAF|snaA]-[gene|A6A13C54177381CE78ABA2CFC2A3F2CE12099F15|tcyJ]-[gene|A8FA40EACDB15CF53DDDA725A3AA5A6EB654FA8B|tcyK]-[gene|350C97E4A4C60A6E2FF9610D50B4A49FB9F9B42D|tcyL]-[gene|B1DD32A3B3D1C0C5391A40834940257357A2F30F|tcyM]-[gene|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN]-[gene|EDC3565D90D7059A4A4C66A13EB568420F6CECD4|cmoO]-[gene|FCD25CA4414D689B217F54B0C44EB805F9590454|cmoI]-[gene|8145952307BC47805A7A9BD39418474358043BFE|cmoJ]-[gene|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR]-[gene|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA]-[gene|910EAAF48C85D0364751EC9585121246AF677431|ytnM]

  description: [Pubmed|15668000]

  regulation

  • induced in the presence of methionine and taurine [Pubmed|11390694]

  regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]: repression, [Pubmed|16109943], in [regulon|protein:50930C56C27D22715620A350220E3C56ADB41020|cymR regulon]
  • [protein|741156D495BE3857683C8A0390764EAD83845ABC|ascR]: activation, [Pubmed|16109943], in [regulon|protein:741156D495BE3857683C8A0390764EAD83845ABC|ascR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|15272571], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

additional information

• [protein|search|translation] is likely to require [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]

Biological materials

Mutant

• GP4474 (trpC2 Δ[gene|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA]::neo), available in [wiki|Jörg Stülke]'s lab
• BKE29290 ([gene|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29290 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AGACATATGTTCCGCTCCTT,  downstream forward: _UP4_GATTGATATAGATGGGGTGT
• BKK29290 ([gene|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29290 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AGACATATGTTCCGCTCCTT,  downstream forward: _UP4_GATTGATATAGATGGGGTGT

References

• 16109943,15668000,15272571,23944997,29626092,35584544,41260340