levR

168

transcriptional activator of the levD-levE-levF-levG-sacC operon

Locus: BSU_27080

Molecular weight: 105.99 kDa

Isoelectric point: 5.94

Protein length: 935 aa

Gene length: 2808 bp

Function: regulation of levan and fructose utilization

Product: transcriptional activator, PRD-type (for SigL-dependent promoter)

Essential: no

Synonyms: levR

Genomic Context

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List of homologs in different organisms, belongs to COG3933 (Galperin et al., 2021)

Gene

Coordinates: 2,763,025  2,765,832

The protein

Catalyzed reaction/ biological activity

• binding to the upstream activating sequence in front of the levD gene results in activation of transcription of the levD-levE-levF-levG-sacC operon, bining occurs in the presence of fructose

Protein family

• Transcription factors activating transcription at SigL-dependent promoters
• PRD-containing transcription factors (according to UniProt)

Domains

• Sigma-54 factor interaction domain (aa 117-348) (according to UniProt)
• 2 PRD domains (aa 468-573, aa 831-935) (according to UniProt)
• PTS EIIA domain type-4 (aa 574-711) (according to UniProt)

Structure

• P23914 (AlphaFold)

Modification

• phosphorylation (His503, His582, His866) (according to Swiss-Prot)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: levR

  Description: PubMed

Biological materials

Mutant

• BKE27080 (levR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTCTATCATCCTTTGTC,  downstream forward: _UP4_TAAAAACAGTTTTTTCATAT
• BKK27080 (levR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTCTATCATCCTTTGTC,  downstream forward: _UP4_TAAAAACAGTTTTTTCATAT

References

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